LOCUS       sequence-162577-         327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of FnR.0.6.2-Y78W protein .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Johnson SM, Javner C, Hackel BJ
  TITLE     Development and Implementation of a Protein–Protein Binding 
            Experiment To Teach Intermolecular Interactions in High School or 
            Undergraduate Classrooms
  JOURNAL   J. Chem. Educ. 2017, 94, 3, 367–374
REFERENCE   2  (bases 1 to 327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Chem. 
            Educ. 2017, 94, 3, 367–374"
COMMENT     Sequence Label: pET - FnR062.Y78W
FEATURES             Location/Qualifiers
     source          1..327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             307..324
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggctagct cctccgactc tccgcgtaac ctggaggtta ccaacgcaac tccgaactct
       61 ctgactattt cttgggacga tccttatcac gctatctatt accgtatcac ctacggcgaa
      121 actggtggta actccccgag ccaggaattc actgttccga gatcttattc taatgcgacc
      181 atcagcggtc tgaaaccggg ccaggattat accattaccg tgtacgctgt aaccggttgg
      241 aacgacgatt caaacccaat cagcatcaat tatcgcaccg aaatcgacaa accgtctcag
      301 ggatcccacc atcaccatca tcactga
//
