LOCUS       sequence-162580-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr1-12GS-I3-01-myc-Late2(delta).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-36 (deltaL)
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(60..89)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(811..830)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(812..830)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(875..927)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(905..925)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cgaactgggt aaatcgcttc catatattca gctgccgcag ctgccaggat cacgcggcgc
       61 aggtcttctt cgctaatcag tttctgttcg gtgcagccac gaatcttctc aacgaacgcc
      121 ttggcctttt ccgccacttc caccggggtg cctttcacta acgcgctgcc cacgcccacg
      181 gccagcacgc ccgctttgaa ccattcgcac acattgtcca gattaacacc gccggttggc
      241 acaaatttca cattcggaaa cggacctttc atcgctttaa caaactgcgg acccaccacc
      301 tcacccggaa acagttttaa gatggtatgg cccagtttca tggctttcac cagttcggtc
      361 ggggtcataa cgcctggcat atagaacaca cccttctcct tgcaaaattg gctaatttcc
      421 tcgtccaggt gcggactcac aatgaattcg gcgccacttt ccaccgcttt acggcactgc
      481 tccacgctgg tcacggtgcc cgcaccaata atcgcgccca tttccttcag aaagctcagt
      541 tccttaatca cggtatccgc atccggcacg gtaaaggtaa tttcaatcag atgaacgccg
      601 cccaggaaaa ccgccagcgc tttctttttc gcttcttcca cactattggc acgcagcacg
      661 gcaacgatct tgtgtttctt gaacagctct tcgatcttgc taccagagtc agagccggag
      721 ccagatttag aaccagacgc acgcgcaccc atggtacctc cggatcgata attctagagg
      781 gcccgtttaa acgctagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa
      841 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag aacctcccac
      901 cgtacacgcc taccgcccat ttgcgtcaat ggggcgg
//
