LOCUS       sequence-162612-        4584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DCAF8, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_DCAF8_WT_V5
FEATURES             Location/Qualifiers
     source          1..4584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1984..2083)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2100..2119)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2101..2119)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2124..2140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2124..2140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2571..3362
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3455..4043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3944..3963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4197..4214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4373..4400)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4492..4578)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgtccagc aaagggagca gcacagatgg cagaacagac ttagctaatg
      241 gaagcctgtc tagcagtcca gaggagatgt ctggagctga agaggggagg gagacatcct
      301 caggcattga agtggaggcc tcagacctga gtttgagctt gactggggat gatggtggcc
      361 ccaaccgcac cagcacagaa agtcgaggca cagacacaga gagctcaggt gaagataagg
      421 actctgacag catggaggac actggtcatt actccattaa tgatgaaaat cgagtccatg
      481 accgctcaga ggaagaggaa gaggaggaag aagaggagga agaagagcag cctcggcgcc
      541 gtgtacagcg caagcgggct aaccgtgacc aggactcatc agatgatgag cgggccctag
      601 aggactgggt gtcctcagaa acatcagctc taccccgacc tcgctggcaa gccctccctg
      661 cccttcggga gcgggagctg ggttcaagtg cccgctttgt ctatgaggcc tgtggggcaa
      721 gagtctttgt gcagcgtttc cgcctgcagc atgggcttga gggccatact ggttgtgtca
      781 ataccctgca ctttaaccag cgcggcacct ggctggccag tggcagcgat gacctgaagg
      841 tggtggtgtg ggattgggta cggcggcagc cagtactgga ctttgagagt ggccacaaaa
      901 gtaatgtgtt ccaggccaag tttcttccta acagtggtga ttctactctg gccatgtgtg
      961 cccgtgacgg gcaggttcga gtagcagaac tgtctgccac acagtgttgc aagaatacaa
     1021 aacgtgtggc ccagcacaag ggagcgtccc acaagttggc actggaacca gactctccct
     1081 gtacgttctt atctgcaggt gaagatgcag ttgttttcac cattgacctg agacaagacc
     1141 gcccagcgtc gaaactggtg gtgacaaaag agaaagagaa gaaagtgggg ctgtatacga
     1201 tctatgtgaa tcctgccaat acccaccagt ttgcagtggg tggacgagat cagtttgtaa
     1261 ggatttatga ccagaggaaa attgatgaga atgagaacaa tggagtactc aagaagttct
     1321 gtcctcatca cctggtgaac agtgagtcca aagcaaacat cacctgtctt gtgtacagcc
     1381 acgacggcac agagctcctg gccagttaca atgatgaaga catttacctc ttcaactcct
     1441 ctcacagtga tggggcccag tatgttaaga gatacaaggg ccacagaaat aatgccacag
     1501 taaaaggcgt caatttctat ggccccaaga gtgagtttgt ggtgagcggt agtgactgtg
     1561 ggcacatctt cctctgggag aaatcatcct gccagattat tcagttcatg gagggggaca
     1621 agggaggcgt ggtaaactgt cttgagcccc accctcacct gcctgtgctg gcaaccagtg
     1681 gcctagacca tgatgtgaag atctgggcac ccacagctga agcttccact gagctgacag
     1741 ggttaaaaga tgtgattaag aagaacaagc gggagcggga tgaagatagc ttgcaccaaa
     1801 ctgacctgtt tgatagtcac atgctgtggt tccttatgca tcacctgaga cagagacgcc
     1861 atcaccggcg ctggcgagaa cctggggttg gggccacaga cgcggactct gatgagtctc
     1921 ccagctcctc agacacatcg gacgaggagg agggccctga ccgggtgcag tgcatgccat
     1981 ctttgccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     2041 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     2101 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     2161 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     2221 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     2281 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     2341 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     2401 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     2461 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     2521 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     2581 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2641 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2701 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2761 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     2821 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2881 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2941 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     3001 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     3061 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     3121 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     3181 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     3241 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     3301 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     3361 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     3421 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3481 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3541 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3601 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3661 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3721 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3781 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3841 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3901 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3961 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4021 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4081 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4141 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4201 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4261 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     4321 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     4381 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     4441 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     4501 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     4561 agcctttcgt tttatttgat gcct
//
