LOCUS       sequence-162621-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for expression of gene by 
            2xCaMV35S enhancer and omega sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB502-omega
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(80..86,87..104,105..110))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(147..271)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(366..711)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(403..423)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga
       61 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
      121 cctaatgcgg cccctcgagg ggccgccata gtgactggat atgttgtgtt ttacagtatt
      181 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      241 tttctcgttc agcttttttg tacaaacttg ttgataactc tagaaattgt aaatagtaat
      301 tgtaatgttg tttgttgttt gttgttgttg gtaattgttg taaaaataca ctggtgattt
      361 cagcgtgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga
      421 tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt
      481 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
      541 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
      601 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
      661 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
      721 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
      781 atgttcacat caatccactt gctttgaaga cgtggttgga acgtcttct
//
