LOCUS       sequence-162626-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            eCFP (no promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB543
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(107..231)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(242..244,245..247,248..760)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(287..308)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(548..567)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     895..916
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
       61 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
      121 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      181 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgataacag
      241 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      301 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      361 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      421 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
      481 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      541 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      601 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      661 caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa
      721 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      781 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      841 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      901 cctgctggag ttcgtgaccg ccgccgggat cactctcg
//
