LOCUS       sequence-162631-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bioluminescent Reporter of Intracellular Calcium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Yang J, Cumberbatch D, Centanni S, Shi SQ, Winder D, Webb D, Johnson
            CH
  TITLE     Coupling optogenetic stimulation with NanoLuc-based luminescence 
            (BRET) Ca++ sensing.
  JOURNAL   Nat Commun. 2016 Oct 27;7:13268. doi: 10.1038/ncomms13268.
  PUBMED    27786307
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-27"; volume: "7:13268. doi"; pages: " 
            10.1038/ncomms13268"
COMMENT     Sequence Label: pT7-CalfluxVTN
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..555)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(802..823)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagcttcct ttcgggcttt gttagcagcc ggatcaagct tacgccagaa tgcgttcgca
       61 cagccgccag ccggtcactc cgttgatggt tactcggaac agcagggagc cgtcggggtt
      121 gatcaggcgc tcgtcgataa ttttgttgcc gttccacagg gtccctgtta cagtgatctt
      181 tttgccgtcg aacacggcga tgccttcata cggccgtccg aaatagtcga tcatgttcgg
      241 cgtaaccccg tcgattacca gtgtgccata gtgcaggatc accttaaagt gatgatcatc
      301 cacagggtac accaccttaa aaattttttc gatctggccc atttggtcgc cgctcagacc
      361 ttcatacggg atgatgacat ggatgtcgat cttcagccca ttttcaccgc tcaggacaat
      421 cctttggatc ggagttacgg acaccccgag attctgaaac aaactggaca cacctccctg
      481 ttcaaggact tggtccaggt tgtagccggc tgtctgtcgc cagtccccaa cgaaatcttc
      541 gagtgtgaag accatgagct cggccagagg ctggacattc tccaccatct tcaggaactc
      601 atcaaagtca atccttccat ttttgtctgt gtctgaatct ccaaatatct catcgggatc
      661 ctcatctgtg agctgttctc cagtaaggcg gatgatgtct ccaaattcct ccctgtcgat
      721 gaaaccattt ccatccttgt caaaaatgcg aaagcactct gatagctctt cttcgctagc
      781 atcttgcatg cgggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      841 ggggtctttg ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg
      901 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc
      961 gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt
//
