LOCUS       sequence-162636-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of SRSF2 mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Kim E, Ilagan JO, Liang Y, Daubner GM, Lee SC, Ramakrishnan A, Li Y,
            Chung YR, Micol JB, Murphy ME, Cho H, Kim MK, Zebari AS, Aumann S, 
            Park CY, Buonamici S, Smith PG, Deeg HJ, Lobry C, Aifantis I, Modis 
            Y, Allain FH, Halene S, Bradley RK, Abdel-Wahab O
  TITLE     SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific 
            Effects on Exon Recognition.
  JOURNAL   Cancer Cell. 2015 May 11;27(5):617-30. doi: 
            10.1016/j.ccell.2015.04.006.
  PUBMED    25965569
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2015.04"; journalName: "Cancer Cell"; date: 
            "2015-05-11- 11"; volume: "27"; issue: "5"; pages: "617-30"
COMMENT     Sequence Label: pRRL_SRSF2_P95H_mCherry
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             707..730
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             731..784
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 accgaatcac cgacctctct ccccaggggg atccgctatg agctacggcc gcccccctcc
       61 cgatgtggag ggtatgacct ccctcaaggt ggacaacctg acctaccgca cctcgcccga
      121 cacgctgagg cgcgtcttcg agaagtacgg gcgcgtcggc gacgtgtaca tcccgcggga
      181 tcgctacacc aaggagtccc gcggcttcgc cttcgttcgc tttcacgaca agcgcgacgc
      241 tgaggacgct atggatgcca tggacggggc cgtgctggac ggccgcgagc tgcgggtgca
      301 aatggcgcgc tacggccgcc acccggactc acaccacagc cgccggggac cgccaccccg
      361 caggtacggg ggcggtggct acggacgccg gagccgcagc cctaggcggc gtcgccgcag
      421 ccgatcccgg agtcggagcc gttccaggtc tcgcagccga tctcgctaca gccgctcgaa
      481 gtctcggtcc cgcactcgtt ctcgatctcg gtcgacctcc aagtccagat ccgcacgaag
      541 gtccaagtcc aagtcctcgt cggtctccag atctcgttcg cggtccaggt cccggtctcg
      601 gtccaggagt cctcccccag tgtccaagag ggaatccaaa tccaggtcgc gatcgaagag
      661 tccccccaag tctcctgaag aggaaggagc ggtgtcctct ggcggcgact acaaagacga
      721 tgacgacaag gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
      781 ccctatggtg agcaagg
//
