LOCUS       sequence-162651-        4347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing LAP3, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_LAP3_WT_V5
FEATURES             Location/Qualifiers
     source          1..4347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1747..1846)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1863..1882)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1864..1882)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1887..1903)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1887..1903)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2334..3125
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3218..3806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3707..3726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3960..3977
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4136..4163)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4255..4341)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gttcttgctg cctcttccgg ctgcggggcg agtagtcgtc cgacgtctgg
      241 ccgtgagacg tttcgggagc cggagtctct ccaccgcaga catgacgaag ggccttgttt
      301 taggaatcta ttccaaagaa aaagaagatg atgtgccaca gttcacaagt gcaggagaga
      361 attttgataa attgttagct ggaaagctga gagagacttt gaacatatct ggaccacctc
      421 tgaaggcagg gaagactcga accttttatg gtctgcatca ggacttcccc agcgtggtgc
      481 tagttggcct cggcaaaaag gcagctggaa tcgacgaaca ggaaaactgg catgaaggca
      541 aagaaaacat cagagctgct gttgcagcgg ggtgcaggca gattcaagac ctggagctct
      601 cgtctgtgga ggtggatccc tgtggagacg ctcaggctgc tgcggaggga gcggtgcttg
      661 gtctctatga atacgatgac ctaaagcaaa aaaagaagat ggctgtgtcg gcaaagctct
      721 atggaagtgg ggatcaggag gcctggcaga aaggagtcct gtttgcttct gggcagaact
      781 tggcacgcca attgatggag acgccagcca atgagatgac gccaaccaga tttgccgaaa
      841 ttattgagaa gaatctcaaa agtgctagta gtaaaaccga ggtccatatc agacccaagt
      901 cttggattga ggaacaggca atgggatcat tcctcagtgt ggccaaagga tctgacgagc
      961 ccccagtctt cttggaaatt cactacaaag gcagccccaa tgcaaacgaa ccacccctgg
     1021 tgtttgttgg gaaaggaatt acctttgaca gtggtggtat ctccatcaag gcttctgcaa
     1081 atatggacct catgagggct gacatgggag gagctgcaac tatatgctca gccatcgtgt
     1141 ctgctgcaaa gcttaatttg cccattaata ttataggtct ggcccctctt tgtgaaaata
     1201 tgcccagcgg caaggccaac aagccggggg atgttgttag agccaaaaac gggaagacca
     1261 tccaggttga taacactgat gctgagggga ggctcatact ggctgatgcg ctctgttacg
     1321 cacacacgtt taacccgaag gtcatcctca atgccgccac cttaacaggt gccatggatg
     1381 tagctttggg atcaggtgcc actggggtct ttaccaattc atcctggctc tggaacaaac
     1441 tcttcgaggc cagcattgaa acaggggacc gtgtctggag gatgcctctc ttcgaacatt
     1501 atacaagaca ggttgtagat tgccagcttg ctgatgttaa caacattgga aaatacagat
     1561 ctgcaggagc atgtacagct gcagcattcc tgaaagaatt cgtaactcat cctaagtggg
     1621 cacatttaga catagcaggc gtgatgacca acaaagatga agttccctat ctacggaaag
     1681 gcatgactgg gaggcccaca aggactctca ttgagttctt acttcgtttc agtcaagaca
     1741 atgcttaccc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     1801 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     1861 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     1921 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     1981 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     2041 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     2101 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     2161 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     2221 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2281 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     2341 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2401 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2461 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2521 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2581 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2641 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2701 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2761 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     2821 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     2881 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     2941 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3001 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3061 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     3121 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3181 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3241 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3301 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3361 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3421 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3481 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3541 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3601 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3661 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3721 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3781 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3841 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3901 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3961 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4021 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4081 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     4141 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4201 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4261 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4321 ctgagccttt cgttttattt gatgcct
//
