LOCUS       sequence-162660-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Calreticulin mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Wijeyesakere SJ, Bedi SK, Huynh D, Raghavan M
  TITLE     The C-Terminal Acidic Region of Calreticulin Mediates 
            Phosphatidylserine Binding and Apoptotic Cell Phagocytosis.
  JOURNAL   J Immunol. 2016 May 1;196(9):3896-909. doi: 
            10.4049/jimmunol.1502122. Epub 2016 Apr 1.
  PUBMED    27036911
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1502122"; journalName: "J Immunol"; date: 
            "2016-05-1- 1"; volume: "196"; issue: "9"; pages: "3896-909"
COMMENT     Sequence Label: pGB1-mCRT(P-domain)
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..250
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLALNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             257..277
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             641..658
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(711..729)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      725..772
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttccctctag aataattttg tttaacttta agaaggagat atacatatgc accatcatca
       61 tcatcattct tctggtgtag atctgcagta caagcttgct ctgaacggta aaaccctgaa
      121 aggtgaaacc accaccgaag ctgttgacgc tgctaccgcg gaaaaagttt tcaaacagta
      181 cgctaacgac aacggtgttg acggtgaatg gacctacgac gacgctacca aaaccttcac
      241 ggtaaccgaa ggtaccgaga acctgtactt ccaatccaat gccccaccca agaagataaa
      301 ggaccctgat gctgccaagc cggaagactg ggatgaacga gccaagatcg atgaccccac
      361 agattccaag cctgaggact gggacaagcc agagcacatc cctgaccctg atgctaagaa
      421 gcctgaggac tgggatgaag agatggatgg agagtgggaa ccaccagtga ttcaaaatcc
      481 tgaatacaag ggcgagtgga aaccacgtca aattgacaac ccagattaca agggtacctg
      541 gatacaccca gaaattgaca accctgaata ctcctagcat tggaagtgga taacggatcc
      601 gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg
      661 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      721 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      781 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      841 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      901 ttcgctttct tccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      961 gggggctccc tttagggttc cgatttagtg ctttacgg
//
