LOCUS       sequence-162663-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the C-terminus of BirA(R118G) 
            and use in proximity-dependent biotin identification (BioID); myc 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Roux KJ, Kim DI, Raida M, Burke B
  TITLE     A promiscuous biotin ligase fusion protein identifies proximal and 
            interacting proteins in mammalian cells.
  JOURNAL   J Cell Biol. 2012 Mar 12.
  PUBMED    22412018
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2012 Mar 12."
COMMENT     Sequence Label: mycBioID-pBABE-puro
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             52..81
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cagtgtggtg gtacgtagga attcggagac ccaagctggc tagccaccat ggaacaaaaa
       61 ctcatctcag aagaggatct cgacaaggac aacaccgtgc ccctgaagct gatcgccctg
      121 ctggccaacg gcgagttcca ctctggcgag cagctgggag agaccctggg aatgagcaga
      181 gccgccatca acaagcacat ccagacactg agagactggg gagtggacgt gttcaccgtg
      241 cctggcaagg gctacagcct gcctgagcct atccagctgc tgaacgccaa gcagatcctg
      301 ggacagctgg atggcggaag cgtggccgtg ctgcctgtga tcgactccac caatcagtac
      361 ctgctggaca gaatcggaga gctgaagtcc ggcgacgcct gcatcgccga gtaccagcag
      421 gctggcagag gaggcagagg acggaagtgg ttcagcccat tcggagccaa cctgtacctg
      481 tccatgttct ggagactgga gcagggacct gctgctgcca tcggactgag tctggtgatc
      541 ggaatcgtga tggccgaggt gctgagaaag ctgggagccg acaaggtgag agtgaagtgg
      601 cctaatgacc tgtacctcca ggaccgcaag ctggctggca tcctggtgga gctgacaggc
      661 aagacaggcg atgccgctca gatcgtgatc ggagccggaa tcaacatggc catgagaaga
      721 gtggaggaga gcgtggtgaa ccagggctgg atcaccctgc aggaggctgg catcaacctg
      781 gaccggaaca ccctggccgc catgctgatc agagagctga gagccgctct ggagctgttc
      841 gagcaggagg gactggctcc ttacctgagc agatgggaga agctggacaa cttcatcaac
      901 agacctgtga agctgatcat cggcgacaag gaaatcttcg gcatctccag agga
//
