LOCUS       sequence-162665-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the C-terminus of BioID2 and use
            in proximity-dependent biotin identification (BioID); a small MCS is
            present downstream of myc tag and BioID2 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux 
            KJ
  TITLE     An improved smaller biotin ligase for BioID proximity labeling.
  JOURNAL   Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844.
  PUBMED    26912792
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2016 Feb 24. pii: mbc.E15-12-0844."
COMMENT     Sequence Label: mycBioID2-pBABE-puro
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..707)
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     CDS             complement(711..740)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      740..749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(818..834)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(850..872)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 agtcactcga ggcttcttct caggctgaac tcgccgctca ggatctcctt gatgccctcc
       61 tcggtcagga tcagggcgcc gcccttctcg ctcaggccca ccagcttgcc ggtgatcttg
      121 ccctcgccca gcagcttcac ctcctcgccc aggtacagca tcttgctctc gatcttgccc
      181 ttgaactcct tgaagctctt ctccttgaac ttcttcaggt tctcgctgat tctcttcagc
      241 accttcagca gcacctcctt tctgtcccag tccttgccgg tgatctcgta cagggtggtg
      301 gctctgtcct tgatctcctc ggggatctct ctctggttca cgttgatgcc gatgcccacg
      361 atcagcttgt ccttgctcag ctcgcacagc acgccgctca ccttcttctc ctggaagtac
      421 acgtcgttgg gccacttcag gctgaagggg atctcggtga tctcctccag ggcctcgctc
      481 acgctcaggc ccagcaccag gggcagctgc agcaggttct cgaactcctt ggggttcagc
      541 aggaagctga agtacaggcc gccctcctgg ctcagccact ttctgcccag gccgcctctg
      601 cccttggtct gtctgtcggc caccagggcg gtgccgtagc tcacgttcca ctccttcagt
      661 ctctcctggg tgctgtccac ctccttcagc cagatcaggt tcttgaagtc gagatcctct
      721 tctgagatga gtttttgttc catggtggct agccagcttg ggtctccgaa ttcctacgta
      781 ccaccacact gggatccggc cggcgcctag agaaggagtg agggctggat aaagggagga
      841 tcgaggcggg gtcgaacgag gaggttcaag ggggagaga
//
