LOCUS       sequence-162669-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of mutant U2AF1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Ilagan JO, Ramakrishnan A, Hayes B, Murphy ME, Zebari AS, Bradley P,
            Bradley RK
  TITLE     U2AF1 mutations alter splice site recognition in hematological 
            malignancies.
  JOURNAL   Genome Res. 2015 Jan;25(1):14-26. doi: 10.1101/gr.181016.114. Epub 
            2014 Sep 29.
  PUBMED    25267526
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.181016.114"; journalName: "Genome Res"; date: "2015-01";
            volume: "25"; issue: "1"; pages: "14-26"
COMMENT     Sequence Label: pRRL_U2AF1_S34Y_mCherry
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(102..155)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(156..179)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
       61 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tagggccggg attctcctcc
      121 acgtcaccgc atgttagaag acttcctctg ccctccttgt cgtcatcgtc tttgtagtcg
      181 ccgccgaatc gcccagatct ttcacgatct ctcgaccgcc tcctgtcacg ctcccgtccg
      241 ccgccacctc caccaccgcc accgccaccg ccacgaccac ggtctctaga ccgagaacga
      301 cgctcccggg atcgggatct tgatctatgc ttcttgcgac ggcggccata cagctcccgc
      361 cgcagctctc tggaaatggg cttcaaatgc atgaagttgc agaagccgcc tcgtgtgcat
      421 tctcccatct catactgacg gcagcaggct tctctgaagt ccgtcacggg tgacagctcg
      481 gcgtggatcg gctgtccatt aaaccaacgg ttattcaagt caatcacagc cttttccgca
      541 tcttcctcac ggcgaaactt gacgtacacg ttccccacca ggtggtctcc caggttgtca
      601 cagacgttca tctcctctac ttccccatac ttctcctcca tttctgtaaa aacctcctca
      661 aaaaactcat catagtgttc ctgcatctcc acatcgctca cggcacagcg caa
//
