LOCUS       sequence-162677-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EPB49 minigene to test splicing event.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Ilagan JO, Ramakrishnan A, Hayes B, Murphy ME, Zebari AS, Bradley P,
            Bradley RK
  TITLE     U2AF1 mutations alter splice site recognition in hematological 
            malignancies.
  JOURNAL   Genome Res. 2015 Jan;25(1):14-26. doi: 10.1101/gr.181016.114. Epub 
            2014 Sep 29.
  PUBMED    25267526
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.181016.114"; journalName: "Genome Res"; date: "2015-01";
            volume: "25"; issue: "1"; pages: "14-26"
COMMENT     Sequence Label: pUB6_EPB49
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             766..807
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             817..834
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
       61 ttctggccgt ttttggcttt tttgttagac gaagcttggt accgagctcg gatccactag
      121 tccagtgtgg tggaattctg cagatgcctg caggtgagtg cctcctggag gggaacaggc
      181 agagcagcag aagctgggtc ctggcacccg aaagtgtcag gggaaggggg gtgtggggcc
      241 acagagttgc ctggtgaaga agaagaagcc ggggtaaggc tgggaaggtc tccctgcccc
      301 gctctggctc actgcggctt tggtctcccc agaacggaga gggccagagg gggaggatgg
      361 accgggggaa ctccctgccc tgtgtgctgg agcagaaggt gaggggcagg ggacacaagc
      421 gcctgtagcg gggcgggagg ctggggagat gccaggcaag tggaatgtgc tgcggggtct
      481 tcctggtgtt gaagatggga gggatggcag ggaagttggg ggagacagag ggcaagatgg
      541 catgaacccc agtggcctct gcaggttgat gtgggaggcc tagggagtcg aaggacagga
      601 tattctgtga gcctaagatt gcccctcccc ccacccccag atctatccct atgaaatgct
      661 agtggtgacc aacaaggggc gaaccaagct gccaccgggg gtggatcgga tgcggcttga
      721 gatccagcac agtggcggcc gctcgagtct agagggccct tcgaaggtaa gcctatccct
      781 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
//
