LOCUS       sequence-162682-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EPB49 minigene to test splicing event.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Ilagan JO, Ramakrishnan A, Hayes B, Murphy ME, Zebari AS, Bradley P,
            Bradley RK
  TITLE     U2AF1 mutations alter splice site recognition in hematological 
            malignancies.
  JOURNAL   Genome Res. 2015 Jan;25(1):14-26. doi: 10.1101/gr.181016.114. Epub 
            2014 Sep 29.
  PUBMED    25267526
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.181016.114"; journalName: "Genome Res"; date: "2015-01";
            volume: "25"; issue: "1"; pages: "14-26"
COMMENT     Sequence Label: pUB6_EPB49
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaagggcc ctctagactc gagcggccgc cactgtgctg gatctcaagc
      121 cgcatccgat ccacccccgg tggcagcttg gttcgcccct tgttggtcac cactagcatt
      181 tcatagggat agatctgggg gtggggggag gggcaatctt aggctcacag aatatcctgt
      241 ccttcgactc cctaggcctc ccacatcaac ctgcagaggc cactggggtt catgccatct
      301 tgccctctgt ctcccccaac ttccctgcca tccctcccat cttcaacacc aggaagaccc
      361 cgcagcacat tccacttgcc tggcatctcc ccagcctccc gccccgctac aggcgcttgt
      421 gtcccctgcc cctcaccttc tgctccagca cacagggcag ggagttcccc cggtccatcc
      481 tccccctctg gccctctccg ttctggggag accaaagccg cagtgagcca gagcggggca
      541 gggagacctt cccagcctta ccccggcttc ttcttcttca ccaggcaact ctgtggcccc
      601 acacccccct tcccctgaca ctttcgggtg ccaggaccca gcttctgctg ctctgcctgt
      661 tcccctccag gaggcactca cctgcaggca tctgcagaat tccaccacac tggactagtg
      721 gatccgagct cggtaccaag cttcgtctaa caaaaaagcc aa
//
