LOCUS       sequence-162689-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red intensiometric genetically encoded Ca2+-indicators for optical 
            imaging 1.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, 
            Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li 
            WH, Campbell RE
  TITLE     Improved Orange and Red Ca Indicators and Photophysical 
            Considerations for Optogenetic Applications.
  JOURNAL   ACS Chem Neurosci. 2013 Mar 19.
  PUBMED    23452507
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2013 Mar 19."
COMMENT     Sequence Label: CMV-R-GECO1.2
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             122..178
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccaccatgg tcgactcatc
      121 acgtcgtaag tggaataagg caggtcacgc agtcagagct ataggtcggc tgagctcacc
      181 cgtggtttcc gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaaggg
      241 gctgaggctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
      301 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
      361 caacgaggac tacaccatcg tggaacagtg cgaacgcgcc gagggccgcc actccaccgg
      421 cggcatggac gagctgtaca agggaggtac aggcgggagt ctggtgagca agggcgagga
      481 ggataacagg gccatcgtca aggagttcat gcgcttcaag ttgcacatgg agggctccgt
      541 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg aggcctttca
      601 gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcatggg acatcctgtc
      661 ccctcagttg atgtacggct ccaaggccta cattaagcac ccagccgaca tccccgacta
      721 cttcaagctg tccttccccg agggcttcag gtgggagcgc gtgatgaact tcgaggacgg
      781 cggcattatt cacgttagcc aggacacctc cctgcaggac ggcgtat
//
