LOCUS       sequence-162692-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EZH2 minigene to test splicing event.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Kim E, Ilagan JO, Liang Y, Daubner GM, Lee SC, Ramakrishnan A, Li Y,
            Chung YR, Micol JB, Murphy ME, Cho H, Kim MK, Zebari AS, Aumann S, 
            Park CY, Buonamici S, Smith PG, Deeg HJ, Lobry C, Aifantis I, Modis 
            Y, Allain FH, Halene S, Bradley RK, Abdel-Wahab O
  TITLE     SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific 
            Effects on Exon Recognition.
  JOURNAL   Cancer Cell. 2015 May 11;27(5):617-30. doi: 
            10.1016/j.ccell.2015.04.006.
  PUBMED    25965569
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2015.04"; journalName: "Cancer Cell"; date: 
            "2015-05-11- 11"; volume: "27"; issue: "5"; pages: "617-30"
COMMENT     Sequence Label: pUB6_EZH2
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaagggcc ctctagactc gagcggccgc cactgtgctg gatcagagga
      121 gctcgaagtt tcatctttct tctcttcttc ttctttatca ttgttctctc cccccgtttc
      181 agtccctgct tccctatcac tgtctgtatc ctttgattcc agcacattaa tggtgggggt
      241 gctgggcctg ctactgttat tgggaagccg tcctcttctg cggcctcctg gacgttttgg
      301 tggggtcttt atccgctcag cggtgagagc agcagcaaac tcctttgctc cctcctacga
      361 aatgaaaaat gtcaacatca gggcaaagtt ctaaaactca ttttgatgga agagaaaaaa
      421 aaatactata taagccaggt tagtcaaaaa atgtatcaca ttatccattt atcacaaata
      481 atcatttgca tgtctaatat acttgttttg atatgctttt gatagtctga aaatttcaga
      541 aagagaaata tgggttctat atattcctag aatggtacaa aaatcaggca agacagttct
      601 atattgtgaa tttgtaaaat atattagcta gttataatta agttaaaata taagaagata
      661 aagtatgaaa tatacttcat gtaataaagg taattatctc tatggacata cagtctattt
      721 aaaagtgaat ctttacaaa
//
