LOCUS       sequence-162695-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Safran M, Kim WY, O'Connell F, Flippin L, Gunzler V, Horner JW, 
            Depinho RA, Kaelin WG
  TITLE     Mouse model for noninvasive imaging of HIF prolyl hydroxylase 
            activity: assessment of an oral agent that stimulates erythropoietin
            production.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jan 3. 103(1):105-10.
  PUBMED    16373502
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jan 3. 103(1):105-10."
COMMENT     Sequence Label: Luciferase-pcDNA3
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(163..181)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggc attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag
      121 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag
      181 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
      241 gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa
      301 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
      361 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
      421 tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt
      481 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa
      541 attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc
      601 acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg
      661 gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc
      721 gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc
      781 aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga
      841 atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt
      901 gaagaagagc tgtttctgag gagccttcag gatt
//
