LOCUS       sequence-162703-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of SRSF2 mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Kim E, Ilagan JO, Liang Y, Daubner GM, Lee SC, Ramakrishnan A, Li Y,
            Chung YR, Micol JB, Murphy ME, Cho H, Kim MK, Zebari AS, Aumann S, 
            Park CY, Buonamici S, Smith PG, Deeg HJ, Lobry C, Aifantis I, Modis 
            Y, Allain FH, Halene S, Bradley RK, Abdel-Wahab O
  TITLE     SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific 
            Effects on Exon Recognition.
  JOURNAL   Cancer Cell. 2015 May 11;27(5):617-30. doi: 
            10.1016/j.ccell.2015.04.006.
  PUBMED    25965569
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2015.04"; journalName: "Cancer Cell"; date: 
            "2015-05-11- 11"; volume: "27"; issue: "5"; pages: "617-30"
COMMENT     Sequence Label: pRRL_SRSF2_P95R_mCherry
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(102..155)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(156..179)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
       61 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tagggccggg attctcctcc
      121 acgtcaccgc atgttagaag acttcctctg ccctccttgt cgtcatcgtc tttgtagtcg
      181 ccgccagagg acaccgctcc ttcctcttca ggagacttgg ggggactctt cgatcgcgac
      241 ctggatttgg attccctctt ggacactggg ggaggactcc tggaccgaga ccgggacctg
      301 gaccgcgaac gagatctgga gaccgacgag gacttggact tggaccttcg tgcggatctg
      361 gacttggagg tcgaccgaga tcgagaacga gtgcgggacc gagacttcga gcggctgtag
      421 cgagatcggc tgcgagacct ggaacggctc cgactccggg atcggctgcg gcgacgccgc
      481 ctagggctgc ggctccggcg tccgtagcca ccgcccccgt acctgcgggg tggcggtccc
      541 cggcggctgt ggtgtgagtc cgggcggcgg ccgtagcgcg ccatttgcac ccgcagctcg
      601 cggccgtcca gcacggcccc gtccatggca tccatagcgt cctcagcgtc gcgcttgtcg
      661 tgaaagcgaa cgaaggcgaa gccgcgggac tccttggtgt agcgatcccg cgggatgtac
      721 acgtcgccga cgcgcccgta cttctcgaag acgcgc
//
