LOCUS       sequence-162709-        4041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MTERFD1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_MTERFD1_WT_V5
FEATURES             Location/Qualifiers
     source          1..4041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1116..1215)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1232..1251)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1233..1251)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1256..1272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1256..1272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1703..2494
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2587..3175
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3076..3095
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3329..3346
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3505..3532)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3624..3710)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3759..3781
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3773..3790
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3774..3790
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3806..3905
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gatatcctct gacaattgct ttctccagtg gggatttaag acttacagga cttcctcctt
       61 atggaatagt tcccagtcta ctagctcaag tagtcaggag aataattctg cccaaagcag
      121 tctgcttcct tccatgaatg aacagtcaca gaagacacaa aatatatcca gctttgattc
      181 tgagctgttt ctagaagaac tggatgaatt gcctccattg tctccaatgc agccaatttc
      241 agaggaagag gctattcaga ttattgcaga ccctccattg ccaccagctt cattcacact
      301 tcgagactat gtggatcatt ctgagactct gcagaagttg gttcttctag gcgtggattt
      361 gtccaagata gaaaaacatc cagaagcagc aaacctcctt ctgagactgg attttgaaaa
      421 agacattaag caaatgcttc tgtttcttaa agatgtgggt atagaggata accaactggg
      481 agcattcctg acaaaaaatc atgcaatttt ctctgaagac cttgaaaatc tgaagaccag
      541 ggtggcttat ctgcattcaa aaaatttcag taaagcagat gttgcacaga tggtcagaaa
      601 agcaccattt ttgctgaact tttcagtgga aagactggat aacagattgg gattttttca
      661 gaaagaactt gaacttagtg tgaagaagac tagagatctg gtagttcgtc tcccaaggct
      721 gctaactgga agtctggaac ccgtgaaaga aaatatgaag gtttatcgtc ttgaacttgg
      781 ttttaaacat aacgaaattc aacatatgat caccagaatc ccaaagatgt taactgcaaa
      841 taaaatgaaa cttaccgaga cgtttgattt tgtgcacaat gtgatgagca ttccccacca
      901 catcattgtc aagttcccac aggtatttaa tacaaggctg tttaaggtca aagaaagaca
      961 cttgtttctt acctatttag gaagagcaca gtatgatcca gcaaaaccta actacatctc
     1021 tttggacaaa ctagtatcta ttcctgatga aatattttgt gaagagattg ccaaagcatc
     1081 agtacaggac tttgaaaaat tcttaaaaac gctttaccca actttcttgt acaaagttgg
     1141 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     1201 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     1261 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     1321 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     1381 gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     1441 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     1501 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     1561 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     1621 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     1681 ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact
     1741 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     1801 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     1861 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     1921 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     1981 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     2041 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     2101 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     2161 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     2221 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     2281 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     2341 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     2401 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     2461 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     2521 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2581 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2641 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2701 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2761 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2821 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2881 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2941 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3001 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3061 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3121 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3181 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3241 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3301 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3361 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     3421 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag
     3481 ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
     3541 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
     3601 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     3661 cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca
     3721 gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac
     3781 gacggccagt cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg
     3841 ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt
     3901 tggcatggct ttgtcagccc aacagatacc cagatggttt aactcagtta agttgaggag
     3961 cctcattaat gctgcacaac tcacaaaacg ttttactaga ccagcaagaa cactgttaca
     4021 tggcttttct gctcagcctc a
//
