LOCUS       sequence-162712-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Haubensak W, Kunwar PS, Cai H, Ciocchi S, Wall NR, Ponnusamy R, Biag
            J, Dong HW, Deisseroth K, Callaway EM, Fanselow MS, Luthi A, 
            Anderson DJ
  TITLE     Genetic dissection of an amygdala microcircuit that gates 
            conditioned fear.
  JOURNAL   Nature. 2010 Nov 11. 468(7321):270-6.
  PUBMED    21068836
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Nov 11. 468(7321):270-6."
COMMENT     Sequence Label: pAAV-EF1a-FLEX-GTB
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..94
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             584..643
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 tctcggcatg gacgagctgt acaagaagct tggtagtgga gagggcagag gaagtctgct
       61 aacatgcggt gacgtcgagg agaatcctgg cccaatcgat gcgcggctgc tgcccgcgct
      121 gctgctgctg ctgctgcccg gtaacgtgac cggtaacggg tccggtaacg gttctttgtc
      181 ccgttgcccc cccggtcagt tccgctgctc ggagccgccc ggtgcccacg gggagtgtta
      241 cccgcaggac tggctgtgcg acggacaccc cgactgcgac gacgggcggg acgagtgggg
      301 ctgcgggacc agcgcgatcc ccgcggtgcc cacggacaac ggcacagagg ctcccactgc
      361 ccctgctcct ggacgtgctc tgccagccag gaatcacggc cgcatgtgga tgctgatcac
      421 tgcagtgctc ctgtgctgcc tggtagctgt gggtggtatc gctgcatggg ggaagtccaa
      481 agcaaaaagc aggtctgaca tcttcagtct tgaaagcgca tccaaggagc tgctggtgcc
      541 tgacaagagc caggcagact tgttctccgc tagcggtagt ggtcaatgta ctaactacgc
      601 tttgttgaaa ctcgctggcg atgttgaaag taaccccggt ccttccggag tgccccaggc
      661 cctgctgttc gtgcccctgc tggtgttccc cctgtgcttc ggca
//
