LOCUS       sequence-162713-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, 
            Crawford GE, Reddy TE, Gersbach CA
  TITLE     CRISPR-Cas9 epigenome editing enables high-throughput screening for 
            functional regulatory elements in the human genome.
  JOURNAL   Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
  PUBMED    28369033
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853."
COMMENT     Sequence Label: pLV-dCas9-KRAB-PGK-HygR
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..21)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(88..282)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             complement(316..336)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aactttgcgt ttctttttcg gaactgatga tttgatttca aatgcagtct ctgaatcagg
       61 atgggtctct tggtgaattt ctctctccac cagccagggc tcttctccct tctccaaccg
      121 gaggatcaca tctggcttag taagctgata acccaaggaa accaggttct tatagttctc
      181 cagcatcaca tttctgtaca ggatctgctg agcagtgtcc agcagcttcc actcctccct
      241 ggtgaagtcc acaaacacat ccttgaaggt caccagtgtc cgggaccagg cagtcagtga
      301 cttagcatcg ctagccacct tcctcttctt cttggggtca gccctgctgt ctccaccgag
      361 ctgagagagg tcgattcttg tttcatagag ccccgtaatt gactgatgaa tcagtgtggc
      421 gtccaggacc tcctttgtag aggtgtaccg ctttctgtct atggtggtgt cgaagtactt
      481 gaaggctgca ggcgcgccca agttggtcag agtaaacaag tggataatgt tttctgcctg
      541 ctccctgatg ggcttatccc tgtgcttatt gtaagcagaa agcaccttat cgaggttagc
      601 gtcggcgagg atcactcttt tggagaattc gcttatttgc tcgatgatct catcaaggta
      661 gtgtttgtgt tgttccacga acagctgctt ctgctcatta tcttcgg
//
