LOCUS       sequence-162717-        3753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TSTA3, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_TSTA3_WT_V5
FEATURES             Location/Qualifiers
     source          1..3753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     404..420
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     404..420
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     425..444
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        425..443
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    461..560
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1524..1623)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1639..1656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1639..1655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1648..1670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1719..1805
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1897..1924
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2083..2100)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2254..2842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2334..2353)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2935..3726)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
       61 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      121 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
      181 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
      241 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gtagcagcga
      301 agtcgaggca tttctgtcct ggctggtcta gaggaggcat gatgatatat ttttatcttg
      361 tgcaatgtaa catcagagat tttgagacac gggccagagc tgccaggaaa cagctatgac
      421 catgtaatac gactcactat aggggatatc agctggatgg caaataatga ttttattttg
      481 actgatagtg acctgttcgt tgcaacaaat tgataagcaa tgctttctta taatgccaac
      541 tttgtacaag aaagttgggc acttccgggc ctgctcgtag ttgtcagtga accaagcaca
      601 ggtctccttc accgcctgct tgaagggtgt gaaccggaag tcgggcaggt aggtcctcag
      661 cttgctgtta ctggctgtct tcttaaactg cccatccgac ttggttgtat caaaggtgac
      721 ttccccatgg aagtccatgg cctccaccac cgcctcggct gcctccttga tggagacctc
      781 atcttcctcg cccacggaga ggatgatggg ctccacttca ttgtactccc gcaggaccca
      841 gataaagagc tgggccaggt ccagcgagta tatgaactgc ctccgcggat tccctgtacc
      901 ccacaccgtc agggccgagc cgctgctctt ggccaggtgc accttgtgga tgaggccagg
      961 cagcacgtgg ccatcctcga tgttgaagtt gtcgtggggc ccgaagacgt tggtggggat
     1021 gacagcggtg aaggtgcagc cgtactgctg gaagtaggcc ctgttctgca cgtcgatcat
     1081 cctcttggca tacgagtacc caaaattgct gttgtgggga ggcccattgt ggatcatggt
     1141 ctcatctatc gggtaggtcg tcttgtcagg gaagatacag gtggacaggc aggacaccac
     1201 cttgcgggcg cccacctcaa aggccgagtg caggacgttg tcgttcatgt gcacgttttt
     1261 cctccagaag tccaaattgt atttgatatt ccggaacagg ccccccacca ttgcagcaag
     1321 atggatgacg tgtgtgggtt ggaccttctc aaacagggcg cgggtctgtg ctgtatccgt
     1381 gagatcggcg tctttagagg agacaaacac ccagtcctct ccaggaagtc cagctccatc
     1441 tgctaccacc ttctggatgg ctttgcctac cagcccagag ccccctgtca ctagaatccg
     1501 catggatccc tggggttcac ccatgccaac ttttttgtac aaagttggca ttataaaaaa
     1561 gcattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     1621 ttggggcccg agcttaagac tggccgtcgt tttacaacgt cgtgactggg aaaacatcca
     1681 tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     1741 cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     1801 caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag
     1861 gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg acggatggcc
     1921 tttttgcgtt tctacaaact cttcctggct agcggtacgc gtattaattg cgttgcgctc
     1981 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2041 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2101 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2161 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2221 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2281 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2341 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2401 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2461 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2521 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2581 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2641 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2701 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2761 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2821 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2881 gtggaacgac gcgtaactca cgttaaggga ttttggtcat gggtggctcg agggttattt
     2941 gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc
     3001 gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac
     3061 gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc
     3121 gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc
     3181 atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa
     3241 tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac
     3301 gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc
     3361 gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc
     3421 tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag
     3481 aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg
     3541 cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt
     3601 caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg
     3661 gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc
     3721 cctcataatg tttaactttg ttttagggcg act
//
