LOCUS       sequence-162718-         590 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, 
            Crawford GE, Reddy TE, Gersbach CA
  TITLE     CRISPR-Cas9 epigenome editing enables high-throughput screening for 
            functional regulatory elements in the human genome.
  JOURNAL   Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
  PUBMED    28369033
REFERENCE   2  (bases 1 to 590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853."
COMMENT     Sequence Label: pLV-dCas9-KRAB-PGK-HygR
FEATURES             Location/Qualifiers
     source          1..590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(96..107)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atcgaacttc cgttgtgtga tcagtttggc gttcagcagc tgccgccaat aatttttcat
       61 tttcttgaca acttcttctg aggggacgtt atcactcttc cctctatttt tatcggatct
      121 tgtcaacact ttattatcaa tagaatcatc tttgagaaaa gactggggca cgatggcatc
      181 cacgtcgtag tcggagagcc gattgatgtc cagttcctga tccacgtaca tgtccctgcc
      241 gttctgcagg tagtacaggt agagcttctc attctgaagc tgggtgtttt caactgggtg
      301 ttccttaagg atttgggacc ccagttcttt tataccctct tcaatcctct tcatcctttc
      361 cctactgttc ttctgtccct tctgggtagt ttggttctct cgggccatct cgataacgat
      421 attctcgggc ttatgccttc ccattacttt gacgagttca tccacgacct taacggtctg
      481 cagtattccc tttttgatag ctgggctacc tgcaagatta gcgatgtgct cgtgaagact
      541 gtccccctgg ccagaaactt gtgctttctg gatgtcctcc ttaaaggtga
//
