LOCUS       sequence-162721-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Sp dCas9-p300-P2A-PuroR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, 
            Crawford GE, Reddy TE, Gersbach CA
  TITLE     CRISPR-Cas9 epigenome editing enables high-throughput screening for 
            functional regulatory elements in the human genome.
  JOURNAL   Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
  PUBMED    28369033
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853."
COMMENT     Sequence Label: pLV-dCas9-p300-P2A-PuroR
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cggttctaga gcgctgccac catggacaag aagtacagca tcggcctggc catcggcacc
       61 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
      121 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
      181 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
      241 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
      301 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
      361 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
      421 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
      481 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
      541 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
      601 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
      661 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
      721 aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
      781 agcaacttcg acctggccga ggatgccaa
//
