LOCUS       sequence-162723-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Sp dCas9-p300-P2A-PuroR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, 
            Crawford GE, Reddy TE, Gersbach CA
  TITLE     CRISPR-Cas9 epigenome editing enables high-throughput screening for 
            functional regulatory elements in the human genome.
  JOURNAL   Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
  PUBMED    28369033
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853."
COMMENT     Sequence Label: pLV-dCas9-p300-P2A-PuroR
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..94
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaagc gacctgccgc
       61 cacaaagaag gctggacagg ctaagaagaa gaaaggatcc attttcaaac cagaagaact
      121 acgacaggca ctgatgccaa ctttggaggc actttaccgt caggatccag aatcccttcc
      181 ctttcgtcaa cctgtggacc ctcagctttt aggaatccct gattactttg atattgtgaa
      241 gagccccatg gatctttcta ccattaagag gaagttagac actggacagt atcaggagcc
      301 ctggcagtat gtcgatgata tttggcttat gttcaataat gcctggttat ataaccggaa
      361 aacatcacgg gtatacaaat actgctccaa gctctctgag gtctttgaac aagaaattga
      421 cccagtgatg caaagccttg gatactgttg tggcagaaag ttggagttct ctccacagac
      481 actgtgttgc tacggcaaac agttgtgcac aatacctcgt gatgccactt attacagtta
      541 ccagaacagg tatcatttct gtgagaagtg tttcaatgag atccaagggg agagcgtttc
      601 tttgggggat gacccttccc agcctcaaac tacaataaat aaagaacaat tttccaagag
      661 aaaaaatgac acactggatc ctgaactgtt tgttgaatgt acagagtgcg gaagaaagat
      721 gcatcagatc tgtgtccttc accatgagat catctggcct gctggattcg tctgtgatgg
      781 ctgtt
//
