LOCUS       sequence-162725-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral SpCas9-gRNA expression vector with 
            DsRed-Express2-P2A-BlastR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, 
            Crawford GE, Reddy TE, Gersbach CA
  TITLE     CRISPR-Cas9 epigenome editing enables high-throughput screening for 
            functional regulatory elements in the human genome.
  JOURNAL   Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
  PUBMED    28369033
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853."
COMMENT     Sequence Label: pLV-U6-gRNA-UbC-DsRed-P2A-Bsr
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..103
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(536..569)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(647..667)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtacgagcgc gccgaggccc gccaccacct gttccaggga tccggcgcaa caaacttctc
       61 tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgaagacct tcaacatctc
      121 tcagcaggat ctggagctgg tggaggtcgc cactgagaag atcaccatgc tctatgagga
      181 caacaagcac catgtcgggg cggccatcag gaccaagact ggggagatca tctctgctgt
      241 ccacattgag gcctacattg gcagggtcac tgtctgtgct gaagccattg ccattgggtc
      301 tgctgtgagc aacgggcaga aggactttga caccattgtg gctgtcaggc acccctactc
      361 tgatgaggtg gacagatcca tcagggtggt cagcccctgt ggcatgtgca gagagctcat
      421 ctctgactat gctcctgact gctttgtgct cattgagatg aatggcaagc tggtcaaaac
      481 caccattgag gaactcatcc ccctcaagta caccaggaac taaagttgat aatagataac
      541 ttcgtatagc atacattata cgaagttatg aattcgatat caagcttatc gataatcaac
      601 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
      661 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
      721 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
      781 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgca
//
