LOCUS       sequence-162726-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral SpCas9-gRNA expression vector with 
            DsRed-Express2-P2A-BlastR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, 
            Crawford GE, Reddy TE, Gersbach CA
  TITLE     CRISPR-Cas9 epigenome editing enables high-throughput screening for 
            functional regulatory elements in the human genome.
  JOURNAL   Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
  PUBMED    28369033
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853."
COMMENT     Sequence Label: pLV-U6-gRNA-UbC-DsRed-P2A-Bsr
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(51..84)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        complement(95..170)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta ataacttcgt
       61 atagcataca ttatacgaag ttattaaaaa aaaagcaccg actcggtgcc actttttcaa
      121 gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac agagacgtac
      181 aaaaaagagc aagaagctaa aaaagattta aaaattattt ttagcgcagt taatggaaca
      241 ggaactaaat ttaccccaaa aatattacgt gaatcaggat ataacgttat tgaggttgaa
      301 gagcatgcat ttgaagatga aacatttaaa aatgttgtaa atccaaatcc agaatttgat
      361 cctgcatgaa aaataccgct tgaatatggt attaaacatg atgcagatat tattattatg
      421 aatgacccag atgctgacag atttggaatg gcaataaaac atgatggtca ttttgtaaga
      481 ttagatggaa atcaaacagg accaatttta attgattgaa aattatcaaa tctaaaacgc
      541 ttaaatagca ttccaaaaaa tccggctcta tattcaagtt ttgtaacaag tgatttgggt
      601 gatagaatcg ctcatgaaaa atatggagtt aatattgtaa aaactttaac tggatttaaa
      661 tgaatgggta gagaaattgc taaagaagaa gataacggat taaattttgt ttttgcttat
      721 gaagaaagt
//
