LOCUS       sequence-162728-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, 
            Crawford GE, Reddy TE, Gersbach CA
  TITLE     CRISPR-Cas9 epigenome editing enables high-throughput screening for 
            functional regulatory elements in the human genome.
  JOURNAL   Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
  PUBMED    28369033
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853."
COMMENT     Sequence Label: pLV-U6-gRNA-UbC-eGFP-P2A-Bsr
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..68
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(501..534)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(612..632)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agggatccgg cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc
       61 ctggaccgaa gaccttcaac atctctcagc aggatctgga gctggtggag gtcgccactg
      121 agaagatcac catgctctat gaggacaaca agcaccatgt cggggcggcc atcaggacca
      181 agactgggga gatcatctct gctgtccaca ttgaggccta cattggcagg gtcactgtct
      241 gtgctgaagc cattgccatt gggtctgctg tgagcaacgg gcagaaggac tttgacacca
      301 ttgtggctgt caggcacccc tactctgatg aggtggacag atccatcagg gtggtcagcc
      361 cctgtggcat gtgcagagag ctcatctctg actatgctcc tgactgcttt gtgctcattg
      421 agatgaatgg caagctggtc aaaaccacca ttgaggaact catccccctc aagtacacca
      481 ggaactaaag ttgataatag ataacttcgt atagcataca ttatacgaag ttatgaattc
      541 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
      601 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
      661 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
      721 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
      781 gctgacgca
//
