LOCUS       sequence-162729-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, 
            Crawford GE, Reddy TE, Gersbach CA
  TITLE     CRISPR-Cas9 epigenome editing enables high-throughput screening for 
            functional regulatory elements in the human genome.
  JOURNAL   Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
  PUBMED    28369033
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853."
COMMENT     Sequence Label: pLV-U6-gRNA-UbC-eGFP-P2A-Bsr
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(58..91)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        complement(102..177)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaata
       61 acttcgtata gcatacatta tacgaagtta ttaaaaaaaa agcaccgact cggtgccact
      121 ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct ctaaaacaga
      181 gacgtacaaa aaagagcaag aagctaaaaa agatttaaaa attattttta gcgcagttaa
      241 tggaacagga actaaattta ccccaaaaat attacgtgaa tcaggatata acgttattga
      301 ggttgaagag catgcatttg aagatgaaac atttaaaaat gttgtaaatc caaatccaga
      361 atttgatcct gcatgaaaaa taccgcttga atatggtatt aaacatgatg cagatattat
      421 tattatgaat gacccagatg ctgacagatt tggaatggca ataaaacatg atggtcattt
      481 tgtaagatta gatggaaatc aaacaggacc aattttaatt gattgaaaat tatcaaatct
      541 aaaacgctta aatagcattc caaaaaatcc ggctctatat tcaagttttg taacaagtga
      601 tttgggtgat agaatcgctc atgaaaaata tggagttaat attgtaaaaa ctttaactgg
      661 atttaaatga atgggtagag aaattgctaa agaagaagat aacggattaa attttgtttt
      721 tgcttatgaa gaaagttatg gatatgtaat tgatgactca gctagagata a
//
