LOCUS       sequence-162732-        3693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing NOSIP, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_NOSIP_WT_V5
FEATURES             Location/Qualifiers
     source          1..3693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(548..647)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(664..683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(665..683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(688..704)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(688..704)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1135..1926
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2019..2607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2508..2527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2761..2778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2937..2964)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3056..3142)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3191..3213
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3205..3222
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3206..3222
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3238..3337
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gctatcgtga gccggcccct caaccctttc acagccaagg ccctctcggg caccagccca
       61 gatgatgtcc aacctgggcc cagtgtgggt cctccaagta aggacaagga caaagtgctg
      121 cccagcttct ggatcccgtc gctgacgccc gaagccaagg ccaccaagct ggagaagccg
      181 tcccgcacgg tgacctgccc catgtcaggg aagcccctgc gcatgtcgga cctgacgccc
      241 gtgcacttca caccgctaga cagctccgtg gaccgcgtgg ggctcatcac ccgcagcgag
      301 cgctacgtgt gtgccgtgac ccgcgacagc ctgagcaacg ccaccccctg cgctgtgctg
      361 cggccctctg gggctgtggt caccctcgaa tgcgtggaga agctgattcg gaaggacatg
      421 gtggaccctg tgactggaga caaactcaca gaccgcgaca tcatcgtgct gcagcggggc
      481 ggtaccggct tcgcgggctc cggagtgaag ctgcaagcgg agaaatcacg gccggtgatg
      541 caggcctgcc caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
      601 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
      661 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
      721 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
      781 accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca
      841 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
      901 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
      961 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     1021 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     1081 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg
     1141 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     1201 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     1261 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     1321 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     1381 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     1441 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     1501 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     1561 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     1621 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     1681 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     1741 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     1801 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     1861 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     1921 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     1981 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2041 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2101 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2161 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2221 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2281 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2341 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2401 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2461 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2521 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2581 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2641 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     2701 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     2761 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     2821 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     2881 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     2941 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     3001 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     3061 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     3121 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     3181 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa
     3241 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
     3301 ttttttataa tgccaacttt gtacaaaaaa gttggcatga cgcggcatgg caagaactgc
     3361 accgcagggg ccgtctacac ctaccacgag aagaagaagg acacagcggc ctcgggctat
     3421 gggacccaga acattcgact gagccgggat gccgtgaagg acttcgactg ctgttgtctc
     3481 tccctgcagc cttgccacga tcctgttgtc accccagatg gctacctgta tgagcgtgag
     3541 gccatcctgg agtacattct gcaccagaag aaggagattg cccggcagat gaaggcctac
     3601 gagaagcagc ggggcacccg gcgcgaggag cagaaggagc ttcagcgggc ggcctcgcag
     3661 gaccatgtgc ggggcttcct ggagaaggag tcg
//
