LOCUS       sequence-162739-        3975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GABPB1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_GABPB1_WT_V5
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     459..475
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     459..475
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     480..499
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        480..498
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    516..615
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1801..1900)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1916..1933)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1916..1932)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1925..1947)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1996..2082
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2174..2201
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2360..2377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2531..3119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2611..2630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3212..3975,1..28))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atacttcggc gatcaccgct tccctcataa tgtttaactt tgttttaggg cgactgccct
       61 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
      121 gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga
      181 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
      241 agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc
      301 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg
      361 aggcatttct gtcctggctg gtctagagga ggcatgatga tatattttta tcttgtgcaa
      421 tgtaacatca gagattttga gacacgggcc agagctgcca ggaaacagct atgaccatgt
      481 aatacgactc actatagggg atatcagctg gatggcaaat aatgatttta ttttgactga
      541 tagtgacctg ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt
      601 acaagaaagt tgggtaaaca gcttctttat tagtctgaag acgagtcata gcttccaact
      661 tctgtctgta ggcctctgct tcctgttctt tctttaggag ctgctgtcga tatttttgtg
      721 cttctcgatt tgcttcatcc agctgtttct gaagagcttc tctctcttct atttctgcag
      781 attccacccg gttttcaatt atttcgatac attgtctctt agctggtggt tcttcactta
      841 taacagtttc ttcagcaatg tctgttgctg gtactgttaa tacttgttgt ccatctggca
      901 tggtcacaat gatgggctga ccaattccac tggttggaat agagtgcaaa tttccaagct
      961 gaattccatc tgtaactatt gtgatgactt gctgaccccc tgaactaact acttgctgaa
     1021 tggcaccatc cacagattct gcagtaacta cttcttctgt ggccactact ggagtttctg
     1081 aagaattgga caatggagca gatgcttcag ctaaggcagc taatgtagct aatactgatg
     1141 tagaagagtt tccaaactga acagcagata cacccgtttc atctgttgcc ttggatgaat
     1201 tttctgaaga taccagacct gttaggttca ccacccctcc aggtccaatg ataaactgtg
     1261 gtgttgcagc atgtattgtc acagtgtcag gactctctgg gtttgtgttg atttggttct
     1321 gcatagcaat ctgtaatatc tctgctaaat cttcatttcc attgtctatt gaaatatcaa
     1381 atgcagtttt acaaaattta ctttgcgtgt gtacatcagc accatatttg attaaaagtt
     1441 ccaccacctc ttgatgattg tgttctgtgg cccaatggag agctgtcatc tttaacatgt
     1501 cctttgcatt gacatcagca ccatgcttaa gtaaaacctc tactatgctg gcatggccct
     1561 cagaagctgc catatgtaat ggtgttcggt ccactttggt tctggcatct ctgctcacac
     1621 cagctcgcag cagtacctct gtggtggaat aatgaccata ctgtgctgct agatgaagtg
     1681 gagaagttcc cagccagtct gtagtaaagg gagctccatt tgccatcaaa atacgaactt
     1741 catcatcttg acctgctcgt gccgcttcta aaagcttctt tcccaaatct accagggaca
     1801 tgccaacttt tttgtacaaa gttggcatta taaaaaagca ttgctcatca atttgttgca
     1861 acgaacaggt cactatcagt caaaataaaa tcattatttg gggcccgagc ttaagactgg
     1921 ccgtcgtttt acaacgtcgt gactgggaaa acatccatgc tagcgttaac gcgagagtag
     1981 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt
     2041 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     2101 aacgttgtga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     2161 catcaaacta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     2221 cctggctagc ggtacgcgta ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2281 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2341 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2401 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2461 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2521 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2581 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2641 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2701 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2761 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2821 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2881 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2941 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3001 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3061 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3121 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg taactcacgt
     3181 taagggattt tggtcatggg tggctcgagg gttatttgcc gactaccttg gtgatctcgc
     3241 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     3301 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     3361 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     3421 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     3481 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     3541 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     3601 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     3661 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
     3721 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
     3781 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     3841 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
     3901 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
     3961 ctgatagttg agtcg
//
