LOCUS       sequence-162757-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-C1
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..770
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc
       61 tgatcaagga gaacatgcac atgaagctgt acatggaagg caccgtgaac aaccaccact
      121 tcaagtgcac caccgaaggg gagggcaagc cctacgaggg cacccagacc cagaggatta
      181 aggtggtgga gggaggcccc ctgccgttcg cattcgacat cctggccacc tgctttatgt
      241 acgggagcaa gaccttcatc aaccacaccc agggcatccc cgatttcttt aagcagtcct
      301 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgcttaccg
      361 ttacccagga caccagcctc caggacggct gcttgatcta caacgtcaag ctcagagggg
      421 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccacca
      481 ccgagaccct gtaccccgct gacggcggcc tggaaggcag atgcgacatg gccctgaagc
      541 tcgtgggcgg gggccacctg cactgcaacc tgaagaccac atacagatcc aagaaacccg
      601 ctaagaacct caagatgccc ggcgtctact ttgtggaccg cagactggaa agaatcaagg
      661 aggccgacaa tgagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
      721 tccctagcaa actggggcac aaacttaatg gcatggacga gctgtacaag tccggactca
      781 gatctcgagc tcaagcttcg aa
//
