LOCUS       sequence-162759-        5550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing LIG1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_LIG1_WT_V5
FEATURES             Location/Qualifiers
     source          1..5550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2950..3049)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3066..3085)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3067..3085)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3090..3106)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3090..3106)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3537..4328
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4421..5009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4910..4929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5163..5180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5339..5366)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5458..5544)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgcagcga agtatcatgt catttttcca ccccaagaaa gagggtaaag
      241 caaagaagcc tgagaaggag gcatccaata gcagcagaga gacggagccc cctccaaagg
      301 cggcactgaa ggagtggaat ggagtggtgt ccgagagtga ctctccggtg aagaggccag
      361 ggaggaaggc ggcccgggtc ctgggcagcg aaggggaaga ggaggatgaa gcccttagcc
      421 ctgctaaagg ccagaagcct gccctggact gctcacaggt ctccccgccc cgtcctgcca
      481 catctcctga gaacaatgct tccctctctg acacctctcc catggacagt tccccatcag
      541 ggattccgaa gcgtcgcaca gctcggaagc agctcccgaa acggaccatt caggaagtcc
      601 tggaagagca gagtgaggac gaggacagag aagccaagag gaagaaggag gaggaagaag
      661 aggagacccc gaaagaaagc ctcacagagg ctgaagtggc cacagagaag gaaggagaag
      721 acggggacca gcccaccacg cctcccaagc ccctaaagac ctccaaagca gagaccccga
      781 cggaaagcgt ttcagagcct gaggtggcca cgaagcagga actgcaggag gaggaagagc
      841 agaccaagcc tccccgcaga gctcccaaga cgctcagcag cttcttcacc ccccggaagc
      901 cagcagtcaa aaaagaagtg aaggaagagg agccaggggc tccaggaaag gagggagctg
      961 ctgagggacc cctggatcca tctggttaca atcctgccaa gaacaactat catcccgtgg
     1021 aagatgcctg ctggaaaccg ggccagaagg ttccttacct ggctgtggcc cggacgtttg
     1081 agaagatcga ggaggtgtct gctcggctcc ggatggtgga gacgctgagc aacttgctgc
     1141 gctccgtggt ggccctgtcg cctccagacc tcctccctgt cctctacctc agcctcaacc
     1201 accttgggcc accccagcag ggcctggagc ttggcgtggg tgatggtgtc cttctcaagg
     1261 cagtggccca ggccacaggt cggcagctgg agtccgtccg ggctgaggca gccgagaaag
     1321 gcgacgtggg gctggtggcc gagaacagcc gcagcaccca gaggctcatg ctgccaccac
     1381 ctccgctcac tgcctccggg gtcttcagca agttccgcga catcgccagg ctcactggca
     1441 gtgcttccac agccaagaag atagacatca tcaaaggcct ctttgtggcc tgccgccact
     1501 cagaagcccg gttcatcgct aggtccctga gcggacggct gcgccttggg ctggcagagc
     1561 agtcggtgct ggctgccctc tcccaggcag tgagcctcac gcccccgggc caagaattcc
     1621 caccagccat ggtggatgct gggaagggca agacagcaga ggccagaaag acgtggctgg
     1681 aggagcaagg catgatcctg aagcagacgt tctgcgaggt tcccgacctg gaccgaatta
     1741 tccccgtgct gctggagcac ggcctggaac gtctcccgga gcactgcaag ctgagcccag
     1801 ggattcccct gaaaccaatg ttggcccatc ccacccgggg catcagcgag gtcctgaaac
     1861 gctttgagga ggcagctttc acctgcgaat acaaatatga cgggcagagg gcacagatcc
     1921 acgccctgga aggcggggag gtgaagatct tcagcaggaa tcaggaagac aacactggga
     1981 agtacccgga catcatcagc cgcatcccca agattaaact cccatcggtc acatccttca
     2041 tcctggacac cgaagccgtg gcttgggacc gggaaaagaa gcagatccag ccattccaag
     2101 tgctcaccac ccgcaaacgc aaggaggtgg atgcgtctga gatccaggtg caggtgtgtt
     2161 tgtacgcctt cgacctcatc tacctcaatg gagagtccct ggtacgtgag cccctttccc
     2221 ggcgccggca gctgctccgg gagaactttg tggagacaga gggcgagttt gtcttcgcca
     2281 cctccctgga caccaaggac atcgagcaga tcgccgagtt cctggagcag tcagtgaaag
     2341 actcctgcga ggggctgatg gtgaagaccc tggatgttga tgccacctac gagatcgcca
     2401 agagatcgca caactggctc aagctgaaga aggactacct tgatggcgtg ggtgacaccc
     2461 tggacctggt ggtgatcggc gcctacctgg gccgggggaa gcgggccggc cggtacgggg
     2521 gcttcctgct ggcctcctac gacgaggaca gtgaggagct gcaggccata tgcaagcttg
     2581 gaactggctt cagtgatgag gagctggagg agcatcacca gagcctcaag gcgctggtgc
     2641 tgcccagccc acgcccttac gtgcggatag atggcgctgt gattcccgac cactggctgg
     2701 accccagcgc tgtgtgggag gtgaagtgcg ctgacctctc cctctctccc atctaccctg
     2761 ctgcgcgggg cctggtggat agtgacaagg gcatctccct tcgcttccct cggtttattc
     2821 gagtccgtga agacaagcag ccggagcagg ccaccaccag tgctcaggtg gcctgtttgt
     2881 accggaagca aagtcagatt cagaaccaac aaggcgagga ctcaggctct gaccctgaag
     2941 atacctactt gccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     3001 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     3061 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     3121 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
     3181 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     3241 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     3301 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     3361 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     3421 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     3481 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
     3541 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     3601 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     3661 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     3721 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     3781 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     3841 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     3901 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     3961 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     4021 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     4081 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     4141 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     4201 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     4261 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     4321 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     4381 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4441 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4501 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4561 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4621 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4681 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4741 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4801 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4861 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4921 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4981 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5041 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5101 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5161 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     5221 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     5281 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     5341 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     5401 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     5461 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     5521 cgactgagcc tttcgtttta tttgatgcct
//
