LOCUS       sequence-162764-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cre-dependent DN-AMPK under EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Kong D, Dagon Y, Campbell JN, Guo Y, Yang Z, Yi X, Aryal P, 
            Wellenstein K, Kahn BB, Sabatini BL, Lowell BB
  TITLE     A Postsynaptic AMPK-->p21-Activated Kinase Pathway Drives 
            Fasting-Induced Synaptic Plasticity in AgRP Neurons.
  JOURNAL   Neuron. 2016 Jun 16. pii: S0896-6273(16)30204-5. doi: 
            10.1016/j.neuron.2016.05.025.
  PUBMED    27321921
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jun 16. pii: S0896-6273(16)30204-5. doi: 
            10.1016/j.neuron.2016.05.025."
COMMENT     Sequence Label: pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    261..294
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    345..378
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     intron          418..621
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(441..461)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
       61 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      121 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      181 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      241 ctgtacaagt aaggcgcgcc ataacttcgt ataatgtatg ctatacgaag ttattaagag
      301 gtttcatatt gctaatagca gctacaatcc agctaccatt ctgcataact tcgtataaag
      361 tatcctatac gaagttattc cggagtcgac tctagaggat ccggtacctc acgacacctg
      421 aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa
      481 ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt
      541 ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc aacctaaaga
      601 cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc ggtactccgt
      661 ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc
      721 acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagg
//
