LOCUS       sequence-162767-        4884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ZBTB24, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ZBTB24_WT_V5
FEATURES             Location/Qualifiers
     source          1..4884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2284..2383)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2400..2419)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2401..2419)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2424..2440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2424..2440)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2871..3662
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3755..4343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4244..4263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4497..4514
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4673..4700)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4792..4878)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggcagaa acatcgccag agccttctgg gcagcttgtt gtacactcag
      241 acgctcacag tgacactgtg ctggccagtt ttgaggatca gaggaagaaa ggcttcctct
      301 gtgacattac tttaatcgtg gagaatgtac atttccgggc ccacaaagcc ttacttgctg
      361 ccagtagtga atacttctca atgatgtttg cagaagaggg ggaaatcggc caatccattt
      421 atatgctgga aggcatggtt gcagacacct ttggtatcct gctggaattt atctacacag
      481 gttatctcca tgccagtgag aaaagtacag aacaaatcct ggctactgct cagttcttaa
      541 aagtctatga cctggtaaag gcttacacag acttccaaaa taatcatagc tccccaaagc
      601 caacaacttt gaacactgct ggtgccccag tggttgttat ctctaataag aaaaacgatc
      661 ctccaaagcg gaaacgggga agaccaaaaa aagtcaatac attgcaggag gagaaatcag
      721 aactggctgc agaggaagaa atacagttaa gagtgaacaa ttcagttcag aatagacaaa
      781 actttgtggt taaaggagac agtggtgtac tgaatgagca aattgcagca aaagaaaagg
      841 aagaatcgga gcctacttgt gagccaagta gagaggagga aatgccagtt gaaaaagatg
      901 agaactatga tcccaagacc gaggatggcc aggcaagcca gagtcgatac agcaagcgga
      961 ggatttggag atccgtcaaa cttaaagatt acaaacttgt tggggatcaa gaggaccatg
     1021 gttcagccaa gaggatctgt ggaaggagaa agcgccctgg aggccctgag gcccgctgta
     1081 aagactgtgg caaggtcttt aagtacaatc actttttagc aatccaccag aggagccaca
     1141 caggggagcg acctttcaaa tgtaatgagt gtggaaaagg ctttgcccag aagcactcgc
     1201 tacaggtcca caccaggatg cacacaggcg agcggccgta cacctgcacc gtgtgcagca
     1261 aggctctgac caccaagcac tcactgctgg agcacatgag cctgcactca ggacagaagt
     1321 cttttacctg tgatcaatgc ggaaaatatt tcagccagaa cagacagcta aagagccatt
     1381 accgagttca tacaggccac tcattaccgg aatgcaaaga ctgccatcgc aaattcatgg
     1441 atgtgtctca gctaaagaaa catctgcgaa cacacacagg tgagaagcca tttacttgtg
     1501 aaatctgtgg caaatctttc acagcaaaga gttctcttca gacccacatc agaatccatc
     1561 gaggagaaaa gccatactcc tgtggcattt gtggcaaatc cttctctgac tccagtgcca
     1621 aaaggagaca ctgcattcta cacactggca agaagccttt ctcctgccct gagtgtaact
     1681 tacagtttgc tcgcttagac aacttgaagg ctcacttgaa aattcatagc aaggagaagc
     1741 atgcttcaga tgccagcagc atttctggca gtagtaatac agaagaggtc aggaatattc
     1801 ttcagctaca gccatatcaa ctctctacct cgggagagca ggaaattcag cttctcgtaa
     1861 ccgattctgt acataacatc aatttcatgc ccggtcctag ccagggaatc agcattgtga
     1921 ctgcagagag ttcccaaaac atgactgcag accaggctgc taatcttacc ctgctcacgc
     1981 agcagccaga gcaactgcag aatttaattc tttcagctca acaggagcaa acagaacaca
     2041 ttcagagcct caatatgatt gaaagccaga tggggccctc acaaacagag ccagtgcacg
     2101 tgatcactct gtccaaggaa acactggaac atcttcatgc ccatcaagag caaacagagg
     2161 agctccattt agctacaagt acttcagatc cagctcagca cctgcagctg acacaggagc
     2221 ctggtccgcc accacccact caccacgtgc cccagccaac gccacttggc caggagcaga
     2281 gcttgccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     2341 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     2401 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     2461 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     2521 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     2581 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     2641 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     2701 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     2761 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     2821 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     2881 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2941 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     3001 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     3061 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     3121 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     3181 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     3241 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     3301 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     3361 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     3421 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     3481 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     3541 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     3601 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     3661 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     3721 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3781 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3841 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3901 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3961 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4021 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4081 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4141 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4201 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4261 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4321 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4381 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4441 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4501 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4561 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     4621 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     4681 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     4741 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     4801 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     4861 agcctttcgt tttatttgat gcct
//
