LOCUS       sequence-162768-        4236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DOK1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_DOK1_WT_V5
FEATURES             Location/Qualifiers
     source          1..4236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     371..387
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     371..387
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     392..411
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        392..410
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    428..527
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1974..2073)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2089..2106)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2089..2105)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2098..2120)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2169..2255
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2347..2374
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2533..2550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2704..3292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2784..2803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3385..4176)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
       61 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
      121 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt
      181 acgaaccgaa caggcttatg tccactgggt tcgtgccttc atccgtttcc acggtgtgcg
      241 tcacccggca accttgggta gcagcgaagt cgaggcattt ctgtcctggc tggtctagag
      301 gaggcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacggg
      361 ccagagctgc caggaaacag ctatgaccat gtaatacgac tcactatagg ggatatcagc
      421 tggatggcaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
      481 taagcaatgc tttcttataa tgccaacttt gtacaagaaa gttggcaagg tagagccctc
      541 tgacttgacc ccagtcttgt cagtccctac tctagagagc ccacagtccc agctccctga
      601 ggccccgctc ttctggacct ggctgtacag ggctgagttg tggcttttga tgccactgcc
      661 agttgcagta ccaggttcag ggaaggctgg gccctggggc ttgggagcaa ggaggggctt
      721 tgtgctccga gggggtggca cagcgtagtc atcagtggca gggttgtagg ggagctcata
      781 gccctcctcc ttcacccgag cttggcacca ccatgcatcc ttgggctccc gaggcagatc
      841 ataaaggccc tggggaggga ctggggccag gccctcaggt tcatcataga tgggatcctc
      901 tttggggtct gtcagcttgg ccttcagcaa ctgctgctgc gcatgctcat acaagtccca
      961 atagagaggt ttcttccgtt gtactccctc tcctgcctga gcagacgtgc tgtccaaggg
     1021 gtctgagtat agggagtcct gggaagggca tggagcaatg cgcagggagt ctaagggctc
     1081 agcatacagg gctgggggac tgtcgaggag ctcttggggg ccaggtgggg aaggcaactt
     1141 cccctctgcc acctcccctt catgggagtc agctctgaga acatcgtgcc cctgtccggc
     1201 ctttccctgg gccttctgcc ggtggatggc agtctcaact gcctggaaga tgtcatttcc
     1261 ctgtgccgtc tggaaggtga aggttccagg gcctgagggg cagcggcggc cggcctcgaa
     1321 agagaacatg accttgtccc ggccatagcg acgcaacaga gtgtagggcc aggacaggag
     1381 tggctccagt atctgactct gggcccccac ggtcaggaga gtcagccttt cagcctccac
     1441 cctcagcacg taggagccat gcaggccaca gcgctcggcg gcctcagtcc tctgcaccgt
     1501 tacccagaat tgggatcctt cccaggtagg gctgtacaag gagttctcca gcatctccag
     1561 ggcagaaagc ttaggtgggt tatcggtagg cgccagagtc cagctgcctt tcggaaaggc
     1621 gtttcggcac agcgtctgca cccaggctgc actggacggc gcgtcggccg ccagcaggtg
     1681 cgagcgctga gcagtgtcca ggcggaaggc agtggcgccg ggctcagggg gggtctccac
     1741 ggtgacgggg gccacactca cacactcagc cagacggatc actttgcagt ccaggcggcg
     1801 cgagctccct cggccacccc cagagctcga ccccttatgg tcaaagaact cgagccgcgc
     1861 tacgccgtgg ggactggccg ggtagagcac ggcccaggtc ttcctccacc tcttggtccc
     1921 aaagcgctga ctctgcaaaa aaagcggccc ttccatcact gctccgtcca tggtgccaac
     1981 ttttttgtac aaagttggca ttataaaaaa gcattgctca tcaatttgtt gcaacgaaca
     2041 ggtcactatc agtcaaaata aaatcattat ttggggcccg agcttaagac tggccgtcgt
     2101 tttacaacgt cgtgactggg aaaacatcca tgctagcgtt aacgcgagag tagggaactg
     2161 ccaggcatca aataaaacga aaggctcagt cggaagactg ggcctttcgt tttatctgtt
     2221 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     2281 tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     2341 ctaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttcctggct
     2401 agcggtacgc gtattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2461 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2521 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2581 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2641 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2701 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2761 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2821 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2881 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2941 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3001 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3061 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3121 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3301 tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgtaactca cgttaaggga
     3361 ttttggtcat gggtggctcg agggttattt gccgactacc ttggtgatct cgcctttcac
     3421 gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
     3481 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
     3541 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
     3601 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
     3661 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
     3721 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
     3781 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
     3841 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
     3901 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
     3961 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
     4021 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa
     4081 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
     4141 ttgagtcgat acttcggcga tcaccgcttc cctcataatg tttaactttg ttttagggcg
     4201 actgccctgc tgcgtaacat cgttgctgct ccataa
//
