LOCUS       sequence-162776-        3084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing S100A13, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_S100A13_WT_V5
FEATURES             Location/Qualifiers
     source          1..3084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     303..319
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     303..319
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     324..343
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        324..342
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    360..459
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(754..853)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(869..886)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(869..885)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(878..900)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      949..1035
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1127..1154
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1313..1330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1484..2072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1564..1583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2165..2956)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
       61 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg
      121 aacaggctta tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg
      181 caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg gctggtctag aggaggcatg
      241 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct
      301 gccaggaaac agctatgacc atgtaatacg actcactata ggggatatca gctggatggc
      361 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat
      421 gctttcttat aatgccaact ttgtacaaga aagttgggta cttcttcctg attttcaggt
      481 ctttcttctt cctgatttcc ttggccagct ccccaatcaa tctccagtac tcattgaact
      541 tgagctccga gtcctgattc acatccaagc tcttcatctt ctcatcaaga gagcccacat
      601 ccttgagcag atggggcaac tgctgggtaa ccagctcttt gaactcgttg acgctgaggc
      661 tatccttccg gccctcctgc cttgcaaagg tgaagaaggt ggtgaccacg gtctcaatgg
      721 actcctctag ctctgtcagt ggttctgctg ccatgccaac ttttttgtac aaagttggca
      781 ttataaaaaa gcattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
      841 aaatcattat ttggggcccg agcttaagac tggccgtcgt tttacaacgt cgtgactggg
      901 aaaacatcca tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga
      961 aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 ctgagtagga caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg
     1081 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg
     1141 acggatggcc tttttgcgtt tctacaaact cttcctggct agcggtacgc gtattaattg
     1201 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1261 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1321 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1381 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1441 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1501 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1561 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1621 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1681 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1741 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1801 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1861 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1921 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1981 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2041 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2101 ctgacgctca gtggaacgac gcgtaactca cgttaaggga ttttggtcat gggtggctcg
     2161 agggttattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca
     2221 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
     2281 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
     2341 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
     2401 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
     2461 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
     2521 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
     2581 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
     2641 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
     2701 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
     2761 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac
     2821 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
     2881 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
     2941 tcaccgcttc cctcataatg tttaactttg ttttagggcg actgccctgc tgcgtaacat
     3001 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     3061 cccgaggcat agactgtacc ccaa
//
