LOCUS       sequence-162778-        4422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ACD, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ACD_WT_V5
FEATURES             Location/Qualifiers
     source          1..4422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1822..1921)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1938..1957)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1939..1957)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1962..1978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1962..1978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2409..3200
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3293..3881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3782..3801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4035..4052
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4211..4238)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4330..4416)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gcctggccgc tgtcagagtg acgccgcgat gagagtaaac gggccagcat
      241 cccgtgcacc agcggggtgg accagcggga gtctgcacac aggcccccga gcaggacgcc
      301 ctcgtgcgca ggcgcggggt gtacgtgggc ggggcctcct cctccggccc cgcccagcga
      361 aggagcttcc tcttccgagg aaaggcgggg cctgggcgcc cgcgggaaac ccgggcccgt
      421 tgcatccgct gggtgtagcc gtggggatgg caggttcggg gaggctggtc ctacggccct
      481 ggattcggga gctgattctg gggtcagaga caccctccag tccacgagcc gggcagctgc
      541 ttgaggtact acaggacgcc gaggccgcgg tcgcgggccc atcccacgcc cctgatacgt
      601 ccgacgtcgg ggccacgctg cttgtgtctg acgggaccca cagtgtccga tgcctggtga
      661 cgcgggaggc cctggacacc tcggactggg aggagaagga gttcggcttc cgcgggacag
      721 agggccggct gctgctgctg caggactgcg gggttcatgt ccaggtcgct gagggcggcg
      781 cgcccgcaga gttctatctc caggtggacc gcttcagcct gctgcccacg gagcagcccc
      841 ggctacgggt gcctggttgc aaccaagact tagatgttca gaaaaagctc tatgactgcc
      901 ttgaggagca cctttcagag tccacctcgt ccaatgcagg cctatcactg tcccagcttc
      961 tggatgaaat gcgggaggac caggagcatc agggggcact cgtgtgcctg gctgaaagct
     1021 gcctgacact ggagggccct tgcacagcac cccctgtcac ccactgggct gcctcacgat
     1081 gcaaggccac gggagaagct gtgtacactg tccccagctc aatgctgtgc atctctgaga
     1141 atgaccagct aattctgagc tctctaggcc cctgtcagag gacacagggc cctgagctgc
     1201 ccccaccaga cccggctctg caggacctat ctctgaccct catagcctct cctccttcct
     1261 cacccagttc ctcaggaacc ccggccttac ccggccacat gtcatccgag gaaagtggta
     1321 ccagcatcag ccttctgcct gccctgtcct tggctgctcc agacccaggg cagagaagca
     1381 gctcccagcc ctcaccagcc atctgctcag cccctgccac cctgaccccc aggtccccac
     1441 acgccagccg tacccccagc tccccactcc agagctgcac tcccagtctc tcaccccgta
     1501 gccatgtccc cagtccacac caggctcttg tgaccaggcc ccagaaacct agcctggagt
     1561 tcaaggagtt tgtagggttg ccctgcaaga atcggccgcc ttttcccagg accggagcta
     1621 ccaggggagc ccaggagccc tgctctgtct gggaaccccc aaagaggcat cgtgatggtt
     1681 ctgccttcca gtatgagtat gagccaccct gcacgtccct ctgtgctcgg gtccaagctg
     1741 ccaggcttcc tccccagctc atggcctggg ccttgcactt tctgatggat gcacagccag
     1801 ggtctgagcc aactccgatg tgcccaactt tcttgtacaa agttggcatt ataagaaagc
     1861 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1921 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     1981 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     2041 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     2101 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     2161 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     2221 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     2281 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     2341 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     2401 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     2461 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     2521 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     2581 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2641 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2701 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     2761 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     2821 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     2881 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     2941 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     3001 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     3061 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     3121 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     3181 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     3241 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3421 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3901 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3961 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4021 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     4081 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     4141 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     4201 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     4261 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     4321 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     4381 ggcccagtct tccgactgag cctttcgttt tatttgatgc ct
//
