LOCUS       sequence-162782-        5469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MVP, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_MVP_WT_V5
FEATURES             Location/Qualifiers
     source          1..5469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2006..2105)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2122..2141)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2123..2141)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2146..2162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2146..2162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2593..3384
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3477..4065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3966..3985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4219..4236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4395..4422)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4514..4600)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4649..4671
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4663..4680
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4664..4680
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4696..4795
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gccctgcacc tccgggctcg gcggaacttc cgggacttca ggggagtgtc ccgccgcact
       61 ggggaggagt ggctggtaac agtgcaggac acagaggccc acgtgccaga tgtccacgag
      121 gaggtgctgg gggttgtgcc catcaccacc ctgggccccc acaactactg cgtgattctc
      181 gaccctgtcg gaccggatgg caagaatcag ctggggcaga agcgcgtggt caagggagag
      241 aagtcttttt tcctccagcc aggagagcag ctggaacaag gcatccagga tgtgtatgtg
      301 ctgtcggagc agcaggggct gctgctgagg gccctgcagc ccctggagga gggggaggat
      361 gaggagaagg tctcacacca ggctggggac cactggctca tccgcggacc cctggagtat
      421 gtgccatctg ccaaagtgga ggtggtggag gagcgccagg ccatccctct agacgagaac
      481 gagggcatct atgtgcagga tgtcaagacc ggaaaggtgc gcgctgtgat tggaagcacc
      541 tacatgctga cccaggacga agtcctgtgg gagaaagagc tgcctcccgg ggtggaggag
      601 ctgctgaaca aggggcagga ccctctggca gacaggggtg agaaggacac agctaagagc
      661 ctccagccct tggcgccccg gaacaagacc cgtgtggtca gctaccgcgt gccccacaac
      721 gctgcggtgc aggtgtacga ctaccgagag aagcgagccc gcgtggtctt cgggcctgag
      781 ctggtgtcgc tgggtcctga ggagcagttc acagtgttgt ccctctcagc tgggcggccc
      841 aagcgtcccc atgcccgccg tgcgctctgc ctgctgctgg ggcctgactt cttcacagac
      901 gtcatcacca tcgaaacggc ggatcatgcc aggctgcaac tgcagctggc ctacaactgg
      961 cactttgagg tgaatgaccg gaaggacccc caagagacgg ccaagctctt ttcagtgcca
     1021 gactttgtag gtgatgcctg caaagccatc gcatcccggg tgcggggggc cgtggcctct
     1081 gtcactttcg atgacttcca taagaactca gcccgcatca ttcgcactgc tgtctttggc
     1141 tttgagacct cggaagcgaa gggccccgat ggcatggccc tgcccaggcc ccgggaccag
     1201 gctgtcttcc cccaaaacgg gctggtggtc agcagtgtgg acgtgcagtc agtggagcct
     1261 gtggatcaga ggacccggga cgccctgcaa cgcagcgtcc agctggccat cgagatcacc
     1321 accaactccc aggaagcggc ggccaagcat gaggctcaga gactggagca ggaagcccgc
     1381 ggccggcttg agcggcagaa gatcctggac cagtcagaag ccgagaaagc tcgcaaggaa
     1441 cttttggagc tggaggctct gagcatggcc gtggagagca ccgggactgc caaggcggag
     1501 gccgagtccc gtgcggaggc agcccggatt gagggagaag ggtccgtgct gcaggccaag
     1561 ctaaaagcac aggccttggc cattgaaacg gaggctgagc tccagagggt ccagaaggtc
     1621 cgagagctgg aactggtcta tgcccgggcc cagctggagc tggaggtgag caaggctcag
     1681 cagctggctg aggtggaggt gaagaagttc aagcagatga cagaggccat aggccccagc
     1741 accatcaggg accttgctgt ggctgggcct gagatgcagg taaaactgct ccagtccctg
     1801 ggcctgaaat caaccctcat caccgatggc tccactccca tcaacctctt caacacagcc
     1861 tttgggctgc tggggatggg gcccgagggt cagcccctgg gcagaagggt ggccagtggg
     1921 cccagccctg gggaggggat atccccccag tctgctcagg cccctcaagc tcctggagac
     1981 aaccacgtgg tgcctgtact gcgctaccca actttcttgt acaaagttgg cattataaga
     2041 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     2101 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2161 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2221 tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag
     2281 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     2341 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     2401 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     2461 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     2521 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     2581 aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     2641 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     2701 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     2761 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     2821 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     2881 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     2941 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     3001 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     3061 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     3121 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     3181 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     3241 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     3301 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     3361 atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg
     3421 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3481 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3541 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3601 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3661 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3721 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3781 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3841 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3901 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3961 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4021 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4081 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4141 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4201 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4261 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4321 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     4381 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     4441 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     4501 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     4561 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     4621 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     4681 cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
     4741 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt tggcatggca
     4801 actgaagagt tcatcatccg catcccccca taccactata tccatgtgct ggaccagaac
     4861 agcaacgtgt cccgtgtgga ggtcgggcca aagacctaca tccggcagga caatgagagg
     4921 gtactgtttg cccccatgcg catggtgacc gtccccccac gtcactactg cacagtggcc
     4981 aaccctgtgt ctcgggatgc ccagggcttg gtgctgtttg atgtcacagg gcaagttcgg
     5041 cttcgccacg ctgacctcga gatccggctg gcccaggacc ccttccccct gtacccaggg
     5101 gaggtgctgg aaaaggacat cacacccctg caggtggttc tgcccaacac tgccctccat
     5161 ctaaaggcgc tgcttgattt tgaggataaa gatggagaca aggtggtggc aggagatgag
     5221 tggcttttcg agggacctgg cacgtacatc ccccggaagg aagtggaggt cgtggagatc
     5281 attcaggcca ccatcatcag gcagaaccag gctctgcggc tcagggcccg caaggagtgc
     5341 tgggaccggg acggcaagga gagggtgaca ggggaagaat ggctggtcac cacagtaggg
     5401 gcgtacctcc cagcggtgtt tgaggaggtt ctggatttgg tggacgccgt catccttacg
     5461 gaaaagaca
//
