LOCUS       sequence-162784-        3999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing STAP2, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_STAP2_WT_V5
FEATURES             Location/Qualifiers
     source          1..3999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1399..1498)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1515..1534)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1516..1534)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1539..1555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1539..1555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1986..2777
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2870..3458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3359..3378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3612..3629
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3788..3815)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3907..3993)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggcctctgcc ctgaggccac cccgtgtccc caagcctaag ggtgtcctgc
      241 cttcacacta ctatgagagc tttctagaga agaaggggcc ctgtgaccgg gattacaaga
      301 agttctgggc aggcctgcag ggtctcacca tttatttcta caatagcaat cgggacttcc
      361 agcacgtgga gaagctcaac ttgggagcat ttgagaaact cacagatgag attccctggg
      421 gaagctcacg tgaccctggc acccacttca gcctgattct ccggaatcag gagatcaagt
      481 tcaaggtaga gaccttggag tgtcgggaaa tgtggaaagg cttcatctta acggtggtgg
      541 agctccgtgt cccgaccgac ttgaccctgc ttcctgggca cctatacatg atgtctgaag
      601 tcttggccaa agaggaggcg cgccgtgcac tggagacacc ctcgtgcttc ctgaaggtga
      661 gccggctgga ggcacaactg ctcctggagc gctaccccga gtgcgggaac ctgctgctgc
      721 ggcccagcgg ggacggcgcc gacggcgtgt cggtcaccac gcggcagatg cacaacggga
      781 cgcacgtggt ccggcattac aaggtgaagc gggagggccc caagtacgtg atcgatgtgg
      841 aacagccgtt ctcttgcacc tccctggacg ccgtggtcaa ctatttcgtg tcgcatacca
      901 aaaaggcgct ggtgccattc ctgttagacg aggactacga gaaggtgcta ggctacgtgg
      961 aagccgataa ggagaatggc gagaatgtgt gggtggcgcc ctccgctccg ggcccaggtc
     1021 ctgcaccctg cacaggtggc cccaagccgc tgtcacctgc gtctagccag gacaagctgc
     1081 ccccactgcc cccactaccg aaccaggaag agaactacgt gacccccatt ggagatggcc
     1141 cagctgttga ctatgagaac caagatgtgg cttcctctag ttggccagtc atcctgaagc
     1201 caaagaagtt gccaaagcct cctgccaagc ttccaaagcc acccgttgga cccaagccag
     1261 agcccaaagt ctttaatggt ggcttgggca ggaagctgcc agtcagttca gcccagcctc
     1321 tcttccccac agccgggctg gcagacatga cggcagagct acagaagaag ctggagaaga
     1381 ggcgggcact ggagcactgc ccaactttct tgtacaaagt tggcattata agaaagcatt
     1441 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     1501 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     1561 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1621 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     1681 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     1741 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     1801 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     1861 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1921 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     1981 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2041 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2101 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2161 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2221 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2281 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2341 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2401 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2461 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2521 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2581 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2641 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2701 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     2761 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     2821 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2881 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3001 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3241 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3481 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3541 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3601 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3661 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3721 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     3781 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     3841 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     3901 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     3961 ccagtcttcc gactgagcct ttcgttttat ttgatgcct
//
