LOCUS       sequence-162785-        3783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ACAA1 used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ACAA1_WT_V5
FEATURES             Location/Qualifiers
     source          1..3783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..57)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(211..799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(291..310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(892..1683)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2114..2130
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2114..2130
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2135..2154
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2135..2153
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2171..2270
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3264..3363)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3379..3396)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3379..3395)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3388..3410)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3459..3545
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3637..3664
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
       61 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      721 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
      841 gaacgacgcg taactcacgt taagggattt tggtcatggg tggctcgagg gttatttgcc
      901 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
      961 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
     1021 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
     1081 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
     1141 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
     1201 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
     1261 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
     1321 gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
     1381 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat
     1441 ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt
     1501 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag
     1561 gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg
     1621 ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct
     1681 cataatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca
     1741 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     1801 ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
     1861 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
     1921 cttacgaacc gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt
     1981 gcgtcacccg gcaaccttgg gtagcagcga agtcgaggca tttctgtcct ggctggtcta
     2041 gaggaggcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     2101 gggccagagc tgccaggaaa cagctatgac catgtaatac gactcactat aggggatatc
     2161 agctggatgg caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
     2221 tgataagcaa tgctttctta taatgccaac tttgtacaag aaagttgggc agttcccagg
     2281 gtattcaaag acggcagcgg ctcccattcc agtcccgatg cacatggaca ccactccgta
     2341 tgccctcttc ccacggcgct tcagctcatt gagcagcgtg atggcctgtc gtgccccagt
     2401 gcagcccagt gggtgcccta aggccactgc accccccagg gggttcacct tctcaggggg
     2461 gagtcgtagc ttctccacac agtaggcagc ctggcttgca aaggcctcat tgatctcgaa
     2521 gatgtccacg tcactcactg tcagcccagc tgtggtagaa ccatctttct tgaaggcagg
     2581 cttcagtttg gccaggccct ccatggtggt gctggggcgg ataccctcat cctgggtcac
     2641 agtgatgctc ctcttggtgc ccttgtcatc atggaccgtg gtggtcacag gcacaatctc
     2701 agcttggaaa cagcccttgc tctgggctct tgctgccttc tgctgggaag ccagggcaaa
     2761 ggtatcctgc ttctcccgtg aaatgccaaa ccgctcagcc acattctcag aggttatccc
     2821 acaggccatg ccaatgtcat aagacccatt tctgatgcca cctgctatgc tggccactgc
     2881 ctgtagcccc gacgaacact gtctattgac agtggacaaa ggcacagtct ccgggatgtc
     2941 actcagaaac tgggcgattc gggccatgat tgccccggcc ccaggctgca gcacatttcc
     3001 gacacagatg tcccccagct gttccggcct cagattcacg tccttgagaa ccgcggtcat
     3061 gactgccgag agaagctcgt cgggggtggt gtccttgaag ccgccgcggc ccgcccggca
     3121 gatggccgtg cgccgcccgt gcaccaccac cacgtccgcg gccgaggcct gcggggcacc
     3181 gctcaggcaa ggcgcggcct gcggcatcca gccggaatcg gccggacccc tcaggtggcc
     3241 cagcactacc tgcagcctct gcatgccaac ttttttgtac aaagttggca ttataaaaaa
     3301 gcattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     3361 ttggggcccg agcttaagac tggccgtcgt tttacaacgt cgtgactggg aaaacatcca
     3421 tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     3481 cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     3541 caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag
     3601 gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg acggatggcc
     3661 tttttgcgtt tctacaaact cttcctggct agcggtacgc gtattaattg cgttgcgctc
     3721 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3781 cgc
//
