LOCUS       sequence-162787-        3642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GTPBP8, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_GTPBP8_WT_V5
FEATURES             Location/Qualifiers
     source          1..3642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..29)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11..29)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(34..50)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(34..50)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             481..1272
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1365..1953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1854..1873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2107..2124
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2283..2310)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2402..2488)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2537..2559
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2551..2568
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2552..2568
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2584..2683
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3536..3635)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
       61 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
      121 ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga
      181 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
      241 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
      301 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
      361 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
      421 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt
      481 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
      541 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
      601 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
      661 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
      721 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
      781 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
      841 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
      901 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
      961 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     1021 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     1081 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     1141 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     1201 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     1261 gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg
     1321 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1381 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1441 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1501 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1561 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1621 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1681 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1741 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1801 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1861 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1921 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1981 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2041 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2101 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2161 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2221 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     2281 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     2341 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     2401 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     2461 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     2521 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     2581 ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
     2641 caatgctttt ttataatgcc aactttgtac aaaaaagttg gcatggcggc gcccgggctg
     2701 cggctgggag cgggaagact ctttgaaatg cctgcggtgc tagagcgact gagccgctat
     2761 aatagcacgt cccaagcttt tgctgaggtg ctgcggctgc cgaagcagca gctgaggaag
     2821 ctgctgtacc cgctgcagga agtagagcgg ttcctcgccc cctacgggag gcaagacctt
     2881 cacctgcgta tctttgaccc aagcccggag gacatagcca gggcggacaa catcttcacg
     2941 gccactgaac ggaaccgcat cgactacgtc agctccgccg tccgtatcga ccacgccccg
     3001 gaccttccgc ggccagaggt gtgttttata ggcagaagca atgttggaaa atcatctcta
     3061 atcaaggctt tattttcact ggcccctgag gttgaagtca gagtctccaa aaaaccagga
     3121 cacacaaaga aaatgaattt tttcaaagtt ggaaaacatt ttacagtggt ggacatgcca
     3181 ggttatggct ttagagcacc tgaagatttt gttgacatgg tagagaccta tctaaaagaa
     3241 cgaaggaact tgaagagaac atttttatta gtggatagcg ttgttggaat tcaaaaaaca
     3301 gacaatattg ccatagaaat gtgtgaagaa tttgcattac cttatgtgat tgtattaaca
     3361 aaaattgaca aatcttccaa gggacatctt ttaaaacaag tgcttcagat ccagaaattt
     3421 gttaacatga aaactcaagg atgttttcct cagttgtttc ctgtaagtgc tgtgaccttt
     3481 tctggaatcc acctgttgag atgctttata gccagtgtaa caggaagtct tgactaccca
     3541 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     3601 caggtcacta tcagtcaaaa taaaatcatt atttgccatc ca
//
