LOCUS       sequence-162790-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  TITLE     pBABE-vectors
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe-puro Ras V12
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(135..668,669..671,672..704))
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     primer_bind     complement(775..791)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(807..829)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 attcctacag cttggggggt gactgggctc cagcagccct tccttccttc cttgcttccg
       61 tccttccttc ctcctccttc cgtctgcacc tccttcctgc atccggcacc tccatgtcct
      121 gagcttgtgc tgcgtcagga gagcacacac ttgcagctca tgcagccggg gccactctca
      181 tcaggagggt tcagcttccg cagcttgtgc tgccggatct cacgcaccaa cgtgtagaag
      241 gcatcctcca ctccctgccg ggtcttggcc gaggtctcga tgtaggggat gccgtagctt
      301 cgggcgaggt cctgagcctg ccgagattcc acagtgcgtg cagccaggtc acacttgttc
      361 cccaccagca ccatgggcac gtcatccgag tccttcaccc gtttgatctg ctccctgtac
      421 tggtggatgt cctcaaaaga cttggtgttg ttgatggcaa acacacacag gaagccctcc
      481 ccggtgcgca tgtactggtc ccgcatggcg ctgtactcct cctggccggc ggtatccagg
      541 atgtccaaca ggcacgtctc cccatcaatg accacctgct tccggtagga atcctctata
      601 gtggggtcgt attcgtccac aaaatggttc tggatcagct ggatggtcag cgcactcttg
      661 cccacaccga cggcgcccac caccaccagc ttatattccg tcatcgctcg gccccggccc
      721 tggcccccca agctgtacca ccacactggg atccggccgg cgcctagaga aggagtgagg
      781 gctggataaa gggaggatcg aggcggggtc gaacgaggag gttcaagggg gagagacggg
      841 gcggatggag gaagaggagg cggaggctta gggtgtacaa agggcttgac ccagggaggg
      901 gggtcaaaag ccaaggcttc ccaggtcacg atgtagggga cctggtctgg gtgtccatgc
      961 gggccaggtg aaaagacctt gatcttaacc tgggtgatga ggtctcggtt aaaggtgccg
     1021 tctcgcggcc atccgacgtt aaaggttggc cattctgca
//
