LOCUS       sequence-162791-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     pBABE-vectors
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe-puro Ras V12
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(42..74,75..77,78..611)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     primer_bind     complement(771..791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tgtggtggta cagcttgggg ggccagggcc ggggccgagc gatgacggaa tataagctgg
       61 tggtggtggg cgccgtcggt gtgggcaaga gtgcgctgac catccagctg atccagaacc
      121 attttgtgga cgaatacgac cccactatag aggattccta ccggaagcag gtggtcattg
      181 atggggagac gtgcctgttg gacatcctgg ataccgccgg ccaggaggag tacagcgcca
      241 tgcgggacca gtacatgcgc accggggagg gcttcctgtg tgtgtttgcc atcaacaaca
      301 ccaagtcttt tgaggacatc caccagtaca gggagcagat caaacgggtg aaggactcgg
      361 atgacgtgcc catggtgctg gtggggaaca agtgtgacct ggctgcacgc actgtggaat
      421 ctcggcaggc tcaggacctc gcccgaagct acggcatccc ctacatcgag acctcggcca
      481 agacccggca gggagtggag gatgccttct acacgttggt gcgtgagatc cggcagcaca
      541 agctgcggaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg
      601 tgctctcctg acgcagcaca agctcaggac atggaggtgc cggatgcagg aaggaggtgc
      661 agacggaagg aggaggaagg aaggacggaa gcaaggaagg aaggaagggc tgctggagcc
      721 cagtcacccc ccaagctgta ggaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg
      781 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      841 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
//
