LOCUS       sequence-162794-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA 
            cassette. The activity of Cas9 can be switched on and off in human 
            cells with 4-hydroxytamoxifen (4-HT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Liu KI, Ramli MN, Woo CW, Wang Y, Zhao T, Zhang X, Yim GR, Chong BY,
            Gowher A, Chua MZ, Jung J, Lee JH, Tan MH
  TITLE     A chemical-inducible CRISPR-Cas9 system for rapid control of genome 
            editing.
  JOURNAL   Nat Chem Biol. 2016 Sep 12. doi: 10.1038/nchembio.2179.
  PUBMED    27618190
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Sep 12. doi: 10.1038/nchembio.2179."
COMMENT     Sequence Label: iCas
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             205..213
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             305..325
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcaaatcagc gagttcagca agagggtgat tctggccgac gcgaacctgg ataaggtcct
       61 cagcgcctac aacaagcacc gagacaaacc catcagggag caggccgaga atatcataca
      121 cctgttcacc ctgacaaatc tgggcgcacc tgcggcattc aaatacttcg ataccaccat
      181 cgacaggaaa aggtacacta gcactaagga ggtgctggat gccaccttga tccaccagtc
      241 cattaccggc ctgtatgaga ccaggatcga cctgagccag cttggaggcg actctagggc
      301 ggacccaaaa aagaaaagga aggtggaatt cgctggagac atgagagctg ccaacctttg
      361 gccaagcccg ctcatgatca aacgctctaa gaagaacagc ctggccttgt ccctgacggc
      421 cgaccagatg gtcagtgcct tgttggatgc tgagcccccc atactctatt ccgagtatga
      481 tcctaccaga cccttcagtg aagcttcgat gatgggctta ctgaccaacc tggcagacag
      541 ggagctggtt cacatgatca actgggcgaa gagggtgcca ggctttgtgg atttgaccct
      601 ccatgatcag gtccaccttc ttgaatgtgc ctggctagag atcctgatga ttggtctcgt
      661 ctggcgctcc atggagcacc cagtgaagct actgtttgct cctaacttgc tcttggacag
      721 gaaccaggga aaatgtgtag agggcatggt ggagatcttc gacatgctgc tggctacatc
      781 atctcggttc cgcatgatga atctgcaggg agaggagttt gtgtgcctca aatctattat
      841 tttgcttaat tctggagtgt acacatttct gtccagcacc ctgaagtctc tggaagagaa
      901 ggaccatatc caccgagtcc tggacaagat cacagacact ttgatccacc tgatggccaa
      961 ggcaggcctg accctgcagc agcagcacca gcggctgg
//
