LOCUS       sequence-162806-       10580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10580)
  AUTHORS   Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP
  TITLE     Efficient generation of transgenic reporter strains and analysis of 
            expression patterns in Caenorhabditis elegans using Library MosSCI.
  JOURNAL   Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.24426.
  PUBMED    27294288
REFERENCE   2  (bases 1 to 10580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2016 Jun 13. doi: 10.1002/dvdy.24426."
COMMENT     Sequence Label: pCFJ150-mbk2
FEATURES             Location/Qualifiers
     source          1..10580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..68)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1894..1911
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1895..1911
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1919..1939
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3088..3112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3573..3593)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     4341..4369
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(4531..4555)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5045..5065)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7131..7142
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      complement(8778..9366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8858..8877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9537..10328,10329..10397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10160..10179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10398..10502)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(10570..10580,1..8)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
       61 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
      121 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
      181 gattgcactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
      241 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
      301 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
      361 ttgggtaacg ccagggtttt ccccagtcac gacgtcacag cacgtgtgtt tttgaacacg
      421 gcgatatgta tctgtagatc atccccaatt ttcaacaatg aatctaaata ctgaactttt
      481 tgtgtaatct ttagtgcaga aaaatgacca aaatcagcga ttttccgatt atacccaaaa
      541 tatctcagct caatgagcac ctataaaaat gtaaaaataa agaaagtagt cgaaccatat
      601 tttgtaaagg ctaaaaaact gaataaattt ttatccatcc agcgcggcgt tacaatccgt
      661 ggcccatgtc acgcacggct ttcaaagaaa aaaaaacagt ctgtttgcgc gacatcgatc
      721 tggcaaaaat cctaaaaacg actatgcgtg gctcacttta tattaatatg aaaaatacaa
      781 acttggtggt gcaggtgctg cagaggccat cgatgaagaa gtggaggcct tgttgtcgat
      841 ctacaaaatt gtatggttgt ttgagatttt ccttgaaacg ctctcaatct tggatatgat
      901 ataaattctg gataagatat gatggatatg atctatgttt gatagagtgc atttgaaagg
      961 ataacggccc tgcagacatt tttgaaaaaa taacaaacat ttaattttct cactcgttta
     1021 ggctattccc ctatttttga tattccttcg cacatatgaa aactactttt tttcgaaact
     1081 gttaactcca gaatttataa atctatagcc cttacttgat tatttattat catggttact
     1141 cacctcatgt ctctttctct tttcaagaag tcttcgttca tatctgttgt ctctttcctc
     1201 cttttgaatc tctgctttgc tcttcgccat tgtttcctga aaataatgta acttgaattg
     1261 tgtaatatac ttttttaatt tgaatttggc ttgtaacgcg gaatcactac gtgcgggatc
     1321 atttcttact agaaaaccca gaaaatgcca tatttcactt atctcggggt catttctaat
     1381 tagaaaagct acaaaaacca tttctaatta cggtgacttt agaactggtc gaaatagcta
     1441 attagaaatg ggtttttgct aattagaggt gactctatgt tatctgagta agcctcgttt
     1501 gtatgtaaac tcacagtact aataaatgca agacacccgg gtttgtctag atatgaaata
     1561 attgaaatat caattctgac agacaataat ggtaatcttg ataaggagtt ccacgcccag
     1621 gagaacacgt tagttttctt gtttttgatt gcgtgcgtta ttttggagaa aaactcgatt
     1681 ttttacaaaa taattttttg aaaggaacac tgttcaataa gttttgtctt ttttctcagt
     1741 tgtgatacgg ttttttattc ttttttgtag ttatacagaa gaccgttacg aaacagactg
     1801 atatcgaaac aaacactgat cttacttgca cttataatac gactcactag tgggcagatc
     1861 ttcgaatgca tcgcgcgcac cgtacgtctc gagtgtaaaa cgacggccag tgaattatca
     1921 actatgtata ataaagttgc aaataaacaa aacattttga tagaaaaata ttttttctga
     1981 atttattctc ggtgagcttt gtgtggaggc ttttgtctga attatgtgtt tgttgatgat
     2041 gaggcgacca gcgcgatttt cagacaacaa aaatcgcgtg gaagatgtaa gacaatcaat
     2101 tgcaactaga aagaagagta atttcatata tattggggac aattggggaa gggacaagtt
     2161 ggtagggaaa agacatgtct gtttttaacg aagagggggg ttcatttgga aaaatgggta
     2221 agaatgtcgt gaaggtttca gcagcgtcac tttttaagcg acgagcttca ccacaatgac
     2281 aacaatatat caaacaatag ctaagaaaaa tagtgatttg atgtagtgca aagaagatgg
     2341 aaagagacaa gggaagaaaa ttagtatgaa atgagggggt ggggtgggga gacagtggct
     2401 gatcagaatg cgacctgcac atctatatgc gaacgatgac aaaaatcgta tatttacaca
     2461 aggttgtcat gattgagttg agacactgtc aatcctgcat ttttttttga aaaaaggaaa
     2521 gataatagtg tgtggaatat taaagagaaa agatccacaa atcaagagaa aaaatttcga
     2581 aagcgctcag acacgagaat cgtcagaaag ttgggaaggg agggattagt tacatcgctg
     2641 tgtgattgaa ctataaatta aggattgggg agtaaaaaaa aatataaaaa tgattttttg
     2701 gagaaaaaag agaataaaaa agagtctcta gtgtacaact acgttgccgc aaaatattat
     2761 tcctttttgt gcatgtcaga acctagaacg tatcaaaaat tcaaggcgcg acagggaaaa
     2821 tgcaatgaag gaatacacaa aaaaaacaga aaaaaagttg gtaaacaaac aagaaattaa
     2881 aaatcccgct gatttggagt cagaaacaag agagacagta gagacaaaaa gatacagaga
     2941 gcaagggtct cggcacgaaa tcggaccgag aagctgctac agagagatgg ctgatgaaaa
     3001 atggaaccgt atcgacactt gtaaaatggt tattatgggc gcagaatggt ggtgaaggtg
     3061 ggaaccgtct ggatgagatg accaggttcc actttgtaca agaaagctgg gtttacttgc
     3121 tggaagtgta cttggtgacg gccttggttc cctcagacac ggcgtgcttg gcaagttctc
     3181 ctgggagaat caaacggaca gcggtttgaa tttcgcggga tgagatcgtt gagcgtttgt
     3241 tgtaatgagc aagacgggaa gcttccgaag cgatgcgttc gaatacatcg ttgacgaagg
     3301 agttcatgat agacatggcc ttggaggaga ctccggtgtc tgggtgaact tgcttgagaa
     3361 cacggtagat gtagacggag tacgattcct tgcgggcatg acgtctcttc tttccgtcct
     3421 ttggcttggc aacgacggtc ttggcggcct tcttggctcc cttggcagat ggctttggtg
     3481 gactagttct ggagcggccg ccaccggtgg agctccaccg atcccccggg catttgtata
     3541 gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt
     3601 ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg
     3661 gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa
     3721 cgcttccatc ttcaatgttg tgtctaattt tgaagttctg aaaatttaaa tcagattagt
     3781 tagttagtaa aatcatgttt aaacttacaa ctttgattcc attcttttgt ttgtctgcca
     3841 tgatgtatac attgtgtgag ttatagttgt attccaattt gtgtccaaga atgtttccat
     3901 cttctttaaa atcaatacct tttaactcga ttctattaac aagggtatca ccttcaaact
     3961 tgacttcagc acctgaaaat ttaaatatgt atggttagtt agtaccgaac tgtttaaact
     4021 tacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt acataacctt
     4081 cgggcatggc actcttgaaa aagtcatgcc gtttcatatg atctgggtat ctcgagaagc
     4141 attgaacacc ataacagaaa gtagtgacaa gtgttggctg aaaatttaaa taatcagggt
     4201 tagttagtat atatatgttt aaacttaccc atggaacagg tagttttcca gtagtgcaaa
     4261 taaatttaag ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa
     4321 atttgtgccc attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa
     4381 gttcttctcc tctagacaca ttgatcctag cagaagcaca ggctgcaggg tgacggtcca
     4441 tcccgctctc ctgggcacaa gacatgggca gcgtgccatc atcctgcgcc gccaccgccg
     4501 gcgggaagcc atggctaagt ctagacatgg agcctgcttt tttgtacaaa cttgtcattc
     4561 tctgtctgaa acattcaatt gattatcgat aaaattaatt aagagagaat acaatgagaa
     4621 aacggaaaat cgagcaggat tttggggaaa gtcggggaat ttttgctatt taagctttct
     4681 ttaacaaaga aactgcattt ttccatgaga ttaccatgaa atttgcgtta aatgggtgaa
     4741 tttataacat ttacggctag aaattaattt taaaaaagag aataaacgaa aaattattcg
     4801 agaaaacgtc tttgaagcag aaatgaaatg aaaaaacaac cgccagcgct gacaaaaatg
     4861 atcaaacaaa cggagtacgg tatacgcggt aagacccaaa tgaattttaa ttcggtgcgc
     4921 ctttaaaaaa tgtatttttt taatttcttt atccgtgggc ccaaatgtat ttttttcttg
     4981 tggtttgatg taaaaattgg gttatattat ttgttttatg gtttaatttt ttaaaaactg
     5041 atatcaactt ttctatacaa agttgatagc ttggcgtaat catggtcata gctgttcctg
     5101 cctaggtgag ctctggtacc ctctagtcaa ggccttaagt gagtcgtatt acgtagcccg
     5161 ggcctagttc tagacattct ctaatgaaaa aatctttcag ttgaaattga aaatgagtta
     5221 aagttggagt ttttattgaa aacagatttc cgtgtgatta gtgtttttag cgagtgtgac
     5281 aggacagcga aaaaatatag aaacaagggg ggaactgaaa agcttaggaa tgcattgaac
     5341 atgagaaggg gaaggggaag gaacaaacta gacaggaatt attggaattt aatcacattt
     5401 ggagtttttt ttctattcga cagaataatt atccagaaca tttttgtatt aaatatttat
     5461 gcatcatatg agtagtcggc tttgttgtgc atgacgagtt tgttatcgac gaaatagaag
     5521 ctgtcagaac gagtctcgtt tggattgttg atcatgtcgt ccactgaaaa agagattagt
     5581 cttttgaatt gtacttttta gaataatgac tcactgagtt gttgagagag ttgagggaac
     5641 tcatagatat gttcacagtt gtttcgtgaa ttcggaatac agaatccgaa ttcaaagtca
     5701 aaacacttca gaaggcgatc tttgaagaag tgacgttcga tcattcggaa atgatggatt
     5761 gggatgtctc ctactttgaa ttccacagtt gctccgaccg ttttgagttt caaaaagttt
     5821 ggagcaaatc tataacgcac gtatcttgcc gactcctgtg gcgactcatc attctcctga
     5881 tcgttctccg gtttggcgat ctcgaaaagc acttgctcag tgtccagatc acggatttgg
     5941 aacttggtga actcgatgtt atagatgttc gcagatgggg agcataagaa tcctaaattt
     6001 atgttttaaa ctgaaatcca aagggagcaa gataccttga gtgattcccg gaagtgctaa
     6061 aacgtcgttc ggagtgattt gagctttctt cgcaagctcc gattccgttg tgattccttg
     6121 ttcggtgctt ggtggtggcc gtggcatctg gaaatatgga aaagttcaac aaaaagaaaa
     6181 gagaaaagaa tgaaatcgga tatcaagagt tagttgagcg gtttctctag ttttctgagt
     6241 ctcacctgcg acgggaaggt cgccgagccg ggtggaatcg atcgttgttg ctcggctttc
     6301 atatcggttt ggttggaagc ggctgaaaac ggaaagaagt ggaagaagga aaagagtgtg
     6361 gtgtgacagg aaaatggtaa ttagagggtg ccaaataacc agctatattt tgtttttttt
     6421 tgaaaacatt tttaaaaaga aaaatacgat aatgatatca gatggatttc cggaaaactg
     6481 gtatgaaaaa tttcaacctt tttgagtaca tgtaatcaaa atacactttg taaattatca
     6541 tttttattga aactccacca tttttctatt tataacgcta ataatttgaa aaagaaacct
     6601 attgcgaacc gcggggtgaa tcccaaaaac gaatgcgttt tggtggagtg attgattcga
     6661 atcgaagaag aaaaagaaga agacgtggaa tagagagctc actcttaacc gagcagcaca
     6721 caccgacaga aaaaaaaatg aaatgaatga gggtcttctt cttcttcttc ttcgaatgat
     6781 tgacagaaat gggaaaaaga ggaagattga gaagggaaaa aggaaggaga aaagaagcag
     6841 aagaagacgt cagagaggag aggaacgagc ggaaaagcag cgggcgcaag tcatagaagt
     6901 agcagagctg gggagaagaa gacactatcc aagaaaggaa tgacgagaga gtatgcaaag
     6961 gggtataggg tgcagacaga ataggaacag aataacagat gatgagccaa gaagagttga
     7021 aaagggcgat gaatttgtca tgtaacttaa tttgggtcaa tttgagcatg atgaattgaa
     7081 atcatccctt gttgggagtt aataaccggt ttgttatcag aaaccctgta atagaagggc
     7141 gccctaactt tgagccaatt catcccggtt tctgtcaaat atatcaaaaa gtggtcaact
     7201 gacaaattgt ttttgatatt ataataaaca ttttatccgt taacaatttt cgaatacttt
     7261 ttacaaggac ttggataaat tggctcaaag acgaagtgag tttgctacca tcatggaatc
     7321 aggaggacat ttcgacaatg tcatataatg tttgaaaaat gattttaaca agggtctcat
     7381 tcgagataag caatgctctg agcaagtggc tcgtcgtctt caagaatctc ttgttgatat
     7441 tcatttggat cctgttttgc acgaagcatg tgccatggat attcagagtt tttgcaaaaa
     7501 agttccgcca ggtcaaacaa tctttttaat ttttatatga tacttcgggt gtatttttct
     7561 tctttttcct gttcattttc gcttttttct cacttttttg taacaaaatc tctcactttt
     7621 ctgtaagtct taacaaaagt tcaattggct cgatcaagta aaatttcggc cgtatgaacg
     7681 ttttcaaagg ttttcatttt tttttaagtt cattcacaat atcatgaatc tctcgtcctt
     7741 tggtttccgg taagtagcga aataatataa gcatacttat aaatgaagga attgaatata
     7801 gaataaggaa agcataacta ccaataacgg aatatagagg taatactgta aatgtactga
     7861 taaccaccat tacagtattc attgaatatg ccacagattg agcaatacta cgatgttttt
     7921 gaggtaccag ttcagaggat ataaaccacg aaattggacc aactccagtt ctgaaatttc
     7981 attattcgat taaatcaaaa tatatttgaa aaacctcacc cataagtata accgtagatg
     8041 atcaatgcaa ctaggcatcc gtatttcatc gggtcaatca gaatacgtat ttttgcgaag
     8101 aatacgaatg caattagagc taaattattg agaaatgtga agagaaggag aagcttgcgt
     8161 cgaccaactc tgtaaactta ttcatacaat tttgtcttca aattttgaac gtttaaaact
     8221 atcttattca atgagttagc gggtttttat agtgagttag ttcacgatta tctaataaaa
     8281 ctatgtccaa acatcctaac tgcgaccgta ttctcactaa ctcgtcttaa aaacgttgag
     8341 attattttaa acccttcttc agtacaacta cagtactgat gcacaaatac aataataata
     8401 atacattcag acatactctg aacaatgtaa cttgtttttg ttatactgtg gtaacattta
     8461 ttgaaatgaa atataacctt acttttcaat cacaattcca ccagtaattg ttccagaaac
     8521 ataagctaaa cccattgcag ttgtactcca ttcagcaatt tctgactcaa catttgcatt
     8581 ttctagaaag aaagtagagg ataaaagaag agcccataat ccgacagggc cctgtgaaat
     8641 tgttatccgc tggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa agaccaggaa ccgtaaaaag
     8761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8821 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8881 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8941 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9001 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9061 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9121 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9181 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9241 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9301 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9361 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9421 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9481 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9541 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9601 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tgaccccagt
     9661 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9721 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9781 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9841 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9901 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9961 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10021 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10081 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10141 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10201 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10261 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10321 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10381 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10441 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10501 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    10561 acctataaaa ataggcgtat
//
