LOCUS       sequence-162807-        3552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HLA-DRA, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_HLA-DRA_WT_V5
FEATURES             Location/Qualifiers
     source          1..3552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(97..196)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(213..232)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(214..232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(237..253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(237..253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             684..1475
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1568..2156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2057..2076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2310..2327
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2486..2513)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2605..2691)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2740..2762
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2754..2771
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2755..2771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2787..2886
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gcctgactgt gggtctggtg ggcatcatta ttgggaccat cttcatcatc aagggattgc
       61 gcaaaagcaa tgcagcagaa cgcagggggc ctctgtgccc aactttcttg tacaaagttg
      121 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
      181 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
      241 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
      301 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
      361 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
      421 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
      481 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
      541 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
      601 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
      661 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
      721 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
      781 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
      841 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
      901 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
      961 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     1021 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     1081 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     1141 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     1201 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     1261 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     1321 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     1381 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     1441 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     1501 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1561 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1621 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1681 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1741 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1801 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1861 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1921 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1981 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2041 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2101 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2161 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2221 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2281 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     2341 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2401 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     2461 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     2521 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     2581 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     2641 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     2701 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
     2761 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
     2821 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
     2881 ttggcatggc cataagtgga gtccctgtgc taggattttt catcatagct gtgctgatga
     2941 gcgctcagga atcatgggct atcaaagaag aacatgtgat catccaggcc gagttctatc
     3001 tgaatcctga ccaatcaggc gagtttatgt ttgactttga tggtgatgag attttccatg
     3061 tggatatggc aaagaaggag acggtctggc ggcttgaaga atttggacga tttgccagct
     3121 ttgaggctca aggtgcattg gccaacatag ctgtggacaa agccaacctg gaaatcatga
     3181 caaagcgctc caactatact ccgatcacca atgtacctcc agaggtaact gtgctcacga
     3241 acagccctgt ggaactgaga gagcccaacg tcctcatctg tttcatagac aagttcaccc
     3301 caccagtggt caatgtcacg tggcttcgaa atggaaaacc tgtcaccaca ggagtgtcag
     3361 agacagtctt cctgcccagg gaagaccacc ttttccgcaa gttccactat ctccccttcc
     3421 tgccctcaac tgaggacgtt tacgactgca gggtggagca ctggggcttg gatgagcctc
     3481 ttctcaagca ctgggagttt gatgctccaa gccctctccc agagactaca gagaacgtgg
     3541 tgtgtgccct gg
//
