LOCUS       sequence-162809-       10145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP
  TITLE     Efficient generation of transgenic reporter strains and analysis of 
            expression patterns in Caenorhabditis elegans using Library MosSCI.
  JOURNAL   Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.24426.
  PUBMED    27294288
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2016 Jun 13. doi: 10.1002/dvdy.24426."
COMMENT     Sequence Label: pCFJ150-mex3
FEATURES             Location/Qualifiers
     source          1..10145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..23
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(61..83)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1909..1926
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1910..1926
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1934..1954
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2668..2692
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3153..3173)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     3921..3949
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(4111..4135)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4625..4645)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6711..6722
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      complement(8358..8946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8438..8457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9117..9908,9909..9977))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9740..9759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9978..10082)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
       61 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
      121 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      181 tgcggcatca gagcagattg cactgagagt gcaccatatg cggtgtgaaa taccgcacag
      241 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
      301 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
      361 tgcaaggcga ttaagttggg taacgccagg gttttcccca gtcacgacgt cacagcacgt
      421 gtgtttttga acacggcgat atgtatctgt agatcatccc caattttcaa caatgaatct
      481 aaatactgaa ctttttgtgt aatctttagt gcagaaaaat gaccaaaatc agcgattttc
      541 cgattatacc caaaatatct cagctcaatg agcacctata aaaatgtaaa aataaagaaa
      601 gtagtcgaac catattttgt aaaggctaaa aaactgaata aatttttatc catccagcgc
      661 ggcgttacaa tccgtggccc atgtcacgca cggctttcaa agaaaaaaaa acagtctgtt
      721 tgcgcgacat cgatctggca aaaatcctaa aaacgactat gcgtggctca ctttatatta
      781 atatgaaaaa tacaaacttg gtggtgcagg tgctgcagag gccatcgatg aagaagtgga
      841 ggccttgttg tcgatctaca aaattgtatg gttgtttgag attttccttg aaacgctctc
      901 aatcttggat atgatataaa ttctggataa gatatgatgg atatgatcta tgtttgatag
      961 agtgcatttg aaaggataac ggccctgcag acatttttga aaaaataaca aacatttaat
     1021 tttctcactc gtttaggcta ttcccctatt tttgatattc cttcgcacat atgaaaacta
     1081 ctttttttcg aaactgttaa ctccagaatt tataaatcta tagcccttac ttgattattt
     1141 attatcatgg ttactcacct catgtctctt tctcttttca agaagtcttc gttcatatct
     1201 gttgtctctt tcctcctttt gaatctctgc tttgctcttc gccattgttt cctgaaaata
     1261 atgtaacttg aattgtgtaa tatacttttt taatttgaat ttggcttgta acgcggaatc
     1321 actacgtgcg ggatcatttc ttactagaaa acccagaaaa tgccatattt cacttatctc
     1381 ggggtcattt ctaattagaa aagctacaaa aaccatttct aattacggtg actttagaac
     1441 tggtcgaaat agctaattag aaatgggttt ttgctaatta gaggtgactc tatgttatct
     1501 gagtaagcct cgtttgtatg taaactcaca gtactaataa atgcaagaca cccgggtttg
     1561 tctagatatg aaataattga aatatcaatt ctgacagaca ataatggtaa tcttgataag
     1621 gagttccacg cccaggagaa cacgttagtt ttcttgtttt tgattgcgtg cgttattttg
     1681 gagaaaaact cgatttttta caaaataatt ttttgaaagg aacactgttc aataagtttt
     1741 gtcttttttc tcagttgtga tacggttttt tattcttttt tgtagttata cagaagaccg
     1801 ttacgaaaca gactgatatc gaaacaaaca ctgatcttac ttgcacttat aatacgactc
     1861 actagtgggc agatcttcga atgcatcgcg cgcaccgtac gtctcgagtg taaaacgacg
     1921 gccagtgaat tatcaactat gtataataaa gttggttgct atggatacca gaatatggag
     1981 agacgacacg aatcgcttca ttcacataca ccaaaatctg tggaatatat cagaactttt
     2041 acaaaatacg ataaatttga tgggtgaggt ggggatcact cgagttttta attataaaga
     2101 ggtgctataa atataaaagg cctctcaatt ctccgttagt tgtgcgtagt agagaggggg
     2161 aaaattggga gttaaacact aggtgagaga tagagagaaa agaggaaatc cttataatat
     2221 atataatttt tgtttaaaac atgagttggt cgtcctatca taatgcaaaa aagaataata
     2281 gagaaagtta cagattttcc aaaaaaacct actgtaggaa tatatattgg gggactgcat
     2341 gggttacata tataaatata tataatatag atatttcaaa aatatgaata acaaataata
     2401 atagttatat ataaatatat gtgatttttt tcgttttttt tgtctaggat tttggggtga
     2461 gaatgatggt gatttttgtg tgttctagta atgtagtaaa gaggtgagaa aatgggaatg
     2521 gagggactcg tacaccgatg aacaaagtag aaaatgggaa aaagagtctt ttaaagaatg
     2581 gaaagatgaa tttggaactc gttgaggatg ggtttttgct agatgagagt ctacacgata
     2641 gtaatggaat gatgaatgga tccacagccc actttgtaca agaaagctgg gtttacttgc
     2701 tggaagtgta cttggtgacg gccttggttc cctcagacac ggcgtgcttg gcaagttctc
     2761 ctgggagaat caaacggaca gcggtttgaa tttcgcggga tgagatcgtt gagcgtttgt
     2821 tgtaatgagc aagacgggaa gcttccgaag cgatgcgttc gaatacatcg ttgacgaagg
     2881 agttcatgat agacatggcc ttggaggaga ctccggtgtc tgggtgaact tgcttgagaa
     2941 cacggtagat gtagacggag tacgattcct tgcgggcatg acgtctcttc tttccgtcct
     3001 ttggcttggc aacgacggtc ttggcggcct tcttggctcc cttggcagat ggctttggtg
     3061 gactagttct ggagcggccg ccaccggtgg agctccaccg atcccccggg catttgtata
     3121 gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt
     3181 ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg
     3241 gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa
     3301 cgcttccatc ttcaatgttg tgtctaattt tgaagttctg aaaatttaaa tcagattagt
     3361 tagttagtaa aatcatgttt aaacttacaa ctttgattcc attcttttgt ttgtctgcca
     3421 tgatgtatac attgtgtgag ttatagttgt attccaattt gtgtccaaga atgtttccat
     3481 cttctttaaa atcaatacct tttaactcga ttctattaac aagggtatca ccttcaaact
     3541 tgacttcagc acctgaaaat ttaaatatgt atggttagtt agtaccgaac tgtttaaact
     3601 tacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt acataacctt
     3661 cgggcatggc actcttgaaa aagtcatgcc gtttcatatg atctgggtat ctcgagaagc
     3721 attgaacacc ataacagaaa gtagtgacaa gtgttggctg aaaatttaaa taatcagggt
     3781 tagttagtat atatatgttt aaacttaccc atggaacagg tagttttcca gtagtgcaaa
     3841 taaatttaag ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa
     3901 atttgtgccc attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa
     3961 gttcttctcc tctagacaca ttgatcctag cagaagcaca ggctgcaggg tgacggtcca
     4021 tcccgctctc ctgggcacaa gacatgggca gcgtgccatc atcctgcgcc gccaccgccg
     4081 gcgggaagcc atggctaagt ctagacatgg agcctgcttt tttgtacaaa cttgtcattc
     4141 tctgtctgaa acattcaatt gattatcgat aaaattaatt aagagagaat acaatgagaa
     4201 aacggaaaat cgagcaggat tttggggaaa gtcggggaat ttttgctatt taagctttct
     4261 ttaacaaaga aactgcattt ttccatgaga ttaccatgaa atttgcgtta aatgggtgaa
     4321 tttataacat ttacggctag aaattaattt taaaaaagag aataaacgaa aaattattcg
     4381 agaaaacgtc tttgaagcag aaatgaaatg aaaaaacaac cgccagcgct gacaaaaatg
     4441 atcaaacaaa cggagtacgg tatacgcggt aagacccaaa tgaattttaa ttcggtgcgc
     4501 ctttaaaaaa tgtatttttt taatttcttt atccgtgggc ccaaatgtat ttttttcttg
     4561 tggtttgatg taaaaattgg gttatattat ttgttttatg gtttaatttt ttaaaaactg
     4621 atatcaactt ttctatacaa agttgatagc ttggcgtaat catggtcata gctgttcctg
     4681 cctaggtgag ctctggtacc ctctagtcaa ggccttaagt gagtcgtatt acgtagcccg
     4741 ggcctagttc tagacattct ctaatgaaaa aatctttcag ttgaaattga aaatgagtta
     4801 aagttggagt ttttattgaa aacagatttc cgtgtgatta gtgtttttag cgagtgtgac
     4861 aggacagcga aaaaatatag aaacaagggg ggaactgaaa agcttaggaa tgcattgaac
     4921 atgagaaggg gaaggggaag gaacaaacta gacaggaatt attggaattt aatcacattt
     4981 ggagtttttt ttctattcga cagaataatt atccagaaca tttttgtatt aaatatttat
     5041 gcatcatatg agtagtcggc tttgttgtgc atgacgagtt tgttatcgac gaaatagaag
     5101 ctgtcagaac gagtctcgtt tggattgttg atcatgtcgt ccactgaaaa agagattagt
     5161 cttttgaatt gtacttttta gaataatgac tcactgagtt gttgagagag ttgagggaac
     5221 tcatagatat gttcacagtt gtttcgtgaa ttcggaatac agaatccgaa ttcaaagtca
     5281 aaacacttca gaaggcgatc tttgaagaag tgacgttcga tcattcggaa atgatggatt
     5341 gggatgtctc ctactttgaa ttccacagtt gctccgaccg ttttgagttt caaaaagttt
     5401 ggagcaaatc tataacgcac gtatcttgcc gactcctgtg gcgactcatc attctcctga
     5461 tcgttctccg gtttggcgat ctcgaaaagc acttgctcag tgtccagatc acggatttgg
     5521 aacttggtga actcgatgtt atagatgttc gcagatgggg agcataagaa tcctaaattt
     5581 atgttttaaa ctgaaatcca aagggagcaa gataccttga gtgattcccg gaagtgctaa
     5641 aacgtcgttc ggagtgattt gagctttctt cgcaagctcc gattccgttg tgattccttg
     5701 ttcggtgctt ggtggtggcc gtggcatctg gaaatatgga aaagttcaac aaaaagaaaa
     5761 gagaaaagaa tgaaatcgga tatcaagagt tagttgagcg gtttctctag ttttctgagt
     5821 ctcacctgcg acgggaaggt cgccgagccg ggtggaatcg atcgttgttg ctcggctttc
     5881 atatcggttt ggttggaagc ggctgaaaac ggaaagaagt ggaagaagga aaagagtgtg
     5941 gtgtgacagg aaaatggtaa ttagagggtg ccaaataacc agctatattt tgtttttttt
     6001 tgaaaacatt tttaaaaaga aaaatacgat aatgatatca gatggatttc cggaaaactg
     6061 gtatgaaaaa tttcaacctt tttgagtaca tgtaatcaaa atacactttg taaattatca
     6121 tttttattga aactccacca tttttctatt tataacgcta ataatttgaa aaagaaacct
     6181 attgcgaacc gcggggtgaa tcccaaaaac gaatgcgttt tggtggagtg attgattcga
     6241 atcgaagaag aaaaagaaga agacgtggaa tagagagctc actcttaacc gagcagcaca
     6301 caccgacaga aaaaaaaatg aaatgaatga gggtcttctt cttcttcttc ttcgaatgat
     6361 tgacagaaat gggaaaaaga ggaagattga gaagggaaaa aggaaggaga aaagaagcag
     6421 aagaagacgt cagagaggag aggaacgagc ggaaaagcag cgggcgcaag tcatagaagt
     6481 agcagagctg gggagaagaa gacactatcc aagaaaggaa tgacgagaga gtatgcaaag
     6541 gggtataggg tgcagacaga ataggaacag aataacagat gatgagccaa gaagagttga
     6601 aaagggcgat gaatttgtca tgtaacttaa tttgggtcaa tttgagcatg atgaattgaa
     6661 atcatccctt gttgggagtt aataaccggt ttgttatcag aaaccctgta atagaagggc
     6721 gccctaactt tgagccaatt catcccggtt tctgtcaaat atatcaaaaa gtggtcaact
     6781 gacaaattgt ttttgatatt ataataaaca ttttatccgt taacaatttt cgaatacttt
     6841 ttacaaggac ttggataaat tggctcaaag acgaagtgag tttgctacca tcatggaatc
     6901 aggaggacat ttcgacaatg tcatataatg tttgaaaaat gattttaaca agggtctcat
     6961 tcgagataag caatgctctg agcaagtggc tcgtcgtctt caagaatctc ttgttgatat
     7021 tcatttggat cctgttttgc acgaagcatg tgccatggat attcagagtt tttgcaaaaa
     7081 agttccgcca ggtcaaacaa tctttttaat ttttatatga tacttcgggt gtatttttct
     7141 tctttttcct gttcattttc gcttttttct cacttttttg taacaaaatc tctcactttt
     7201 ctgtaagtct taacaaaagt tcaattggct cgatcaagta aaatttcggc cgtatgaacg
     7261 ttttcaaagg ttttcatttt tttttaagtt cattcacaat atcatgaatc tctcgtcctt
     7321 tggtttccgg taagtagcga aataatataa gcatacttat aaatgaagga attgaatata
     7381 gaataaggaa agcataacta ccaataacgg aatatagagg taatactgta aatgtactga
     7441 taaccaccat tacagtattc attgaatatg ccacagattg agcaatacta cgatgttttt
     7501 gaggtaccag ttcagaggat ataaaccacg aaattggacc aactccagtt ctgaaatttc
     7561 attattcgat taaatcaaaa tatatttgaa aaacctcacc cataagtata accgtagatg
     7621 atcaatgcaa ctaggcatcc gtatttcatc gggtcaatca gaatacgtat ttttgcgaag
     7681 aatacgaatg caattagagc taaattattg agaaatgtga agagaaggag aagcttgcgt
     7741 cgaccaactc tgtaaactta ttcatacaat tttgtcttca aattttgaac gtttaaaact
     7801 atcttattca atgagttagc gggtttttat agtgagttag ttcacgatta tctaataaaa
     7861 ctatgtccaa acatcctaac tgcgaccgta ttctcactaa ctcgtcttaa aaacgttgag
     7921 attattttaa acccttcttc agtacaacta cagtactgat gcacaaatac aataataata
     7981 atacattcag acatactctg aacaatgtaa cttgtttttg ttatactgtg gtaacattta
     8041 ttgaaatgaa atataacctt acttttcaat cacaattcca ccagtaattg ttccagaaac
     8101 ataagctaaa cccattgcag ttgtactcca ttcagcaatt tctgactcaa catttgcatt
     8161 ttctagaaag aaagtagagg ataaaagaag agcccataat ccgacagggc cctgtgaaat
     8221 tgttatccgc tggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8281 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa agaccaggaa ccgtaaaaag
     8341 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8401 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8461 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8521 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8581 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8641 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8701 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8761 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8821 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8881 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8941 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9001 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9061 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tgaccccagt
     9241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9421 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9721 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9781 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10021 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10081 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    10141 accta
//
