LOCUS       sequence-162821-       10828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, 
            Erdjument-Bromage H, Tempst P, Sabatini DM
  TITLE     Rictor, a novel binding partner of mTOR, defines a 
            rapamycin-insensitive and raptor-independent pathway that regulates 
            the cytoskeleton.
  JOURNAL   Curr Biol 2004 Jul 27;14(14):1296-302.
  PUBMED    15268862
REFERENCE   2  (bases 1 to 10828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol";
            date: "2004-07-27"; volume: "14(14)"; pages: "1296-302"
COMMENT     Sequence Label: myc-Raptor
FEATURES             Location/Qualifiers
     source          1..10828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(91..96,97..114,115..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     134..156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     153..169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        198..577
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        578..781
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     732..752
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     778..802
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1012..1030
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1012..1029
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1043..1062
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1147..1156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1156..1185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      7312..7321
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7325..7459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7362..7381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7416..7435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7528..7885
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7528..7719
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7551..7571)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7736..7871
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7905..7922)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7905..7921)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7914..7936)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8134..8589
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8221..8240)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8431..8452
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8606..8625)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8725..8747
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8785..8803)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8871..8975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8976..9044,9045..9836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9194..9213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10007..10595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10496..10515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10749..10766
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      181 ttaattcgag ctcgcccgac attgattatt gactagttat taatagtaat caattacggg
      241 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      301 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      361 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      421 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      481 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      541 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      601 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      661 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      721 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      781 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      841 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
      901 ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc
      961 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
     1021 acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
     1081 ctgcacctcg gttctatcga ttgaattccc gaaccgacag tcggtctctt caccaaggcc
     1141 attcgcgcca ccatggagca aaagctcatt tctgaggaag atctcaatgg tggtggtggt
     1201 gggtcgacga tggagtccga aatgctgcaa tcgcctcttc tgggcctggg ggaggaagat
     1261 gaggctgatc ttacagactg gaacctacct ttggctttta tgaaaaagag gcactgtgag
     1321 aaaattgaag gctccaaatc cttagctcag agctggagga tgaaggatcg gatgaagaca
     1381 gtcagtgttg ccttagtttt gtgcctgaat gttggtgtgg accctcccga tgtggtgaag
     1441 accacgccct gtgcacgctt ggaatgctgg atcgatcctc tgtcgatggg tcctcagaaa
     1501 gctctggaaa ccatcggtgc aaatttacag aagcagtacg agaactggca gccaagggcc
     1561 cggtacaagc agagccttga cccaactgtg gatgaagtca agaagctctg cacgtcctta
     1621 cgtcgcaacg ccaaggagga gcgagtcctc tttcactaca atggccacgg ggtgccccgg
     1681 cccacagtca acggggaggt ctgggtcttc aacaagaact acacgcagta catccctctg
     1741 tccatatatg acctgcagac gtggatgggc agcccgtcga tcttcgtcta cgactgctcc
     1801 aatgctggct tgatcgtcaa gtccttcaag cagttcgcac tacagcggga gcaggagctg
     1861 gaggtagctg caatcaaccc aaatcaccct cttgctcaga tgcctttgcc tccgtcgatg
     1921 aaaaactgca tccagctggc agcctgcgag gccaccgagc tgctgcccat gatccccgac
     1981 ctcccggctg acctattcac ctcctgcctc accaccccca tcaagatcgc cctgcgctgg
     2041 ttttgcatgc agaaatgtgt cagtctggtg cctggcgtca cactggattt gatagaaaag
     2101 atccctggcc gcctgaacga caggaggacg cccctgggtg aactgaactg gatcttcaca
     2161 gccatcacag acaccatcgc gtggaacgtg ctcccccggg atctcttcca aaagctcttc
     2221 agacaggact tgctggtggc tagtctgttt cgaaattttt tattggcgga aaggattatg
     2281 aggtcgtata actgcactcc cgtcagcagc ccgcgtctgc cgcccacgta catgcacgcc
     2341 atgtggcaag cctgggacct ggctgttgac atctgtctgt ctcagctgcc gacgatcatc
     2401 gaggaaggca ctgcgtttcg gcacagcccg ttcttcgccg agcagctgac cgcattccag
     2461 gtgtggctca ccatgggcgt ggagaaccga aacccacccg aacagctgcc catcgtcctg
     2521 caggtgctgt taagccaagt gcaccggctg agagcattgg acttgcttgg aagatttttg
     2581 gacctgggtc cctgggcagt gagcctggcc ttgtctgtcg gcatcttccc ctacgtgctg
     2641 aagctgctcc agagctcggc ccgagagctg cggccacttc tcgttttcat ctgggccaag
     2701 atcctcgcag tggacagctc gtgccaggcg gacctcgtga aggacaacgg ccacaagtac
     2761 ttcctgtcgg tcctggcgga cccctacatg ccagctgaac accggaccat gacggctttc
     2821 attctcgccg tgatcgtcaa cagctatcac acggggcagg aagcctgcct tcagggaaac
     2881 ctcattgcca tctgcctgga gcagctcaac gacccgcacc ccttgctgcg ccagtgggtg
     2941 gccatctgcc tcggcaggat ctggcagaac ttcgactcgg cgaggtggtg cggcgtgagg
     3001 gacagcgctc atgagaagct ctacagcctc ctctccgacc ccattcccga ggtccgctgc
     3061 gcagcggtct tcgcccttgg cacgttcgtg ggcaactctg cagagaggac ggaccactcc
     3121 accaccatcg accacaacgt ggccatgatg ctggcccagc tggtcagcga cgggagcccc
     3181 atggtccgga aggagctggt ggtggctctg agtcatcttg tggttcagta tgaaagcaat
     3241 ttctgcaccg tggccctgca gttcatagaa gaggaaaaga actacgcctt gccttctcca
     3301 gcaaccacag agggagggag tttgacccca gtgcgagaca gcccgtgcac ccccagactt
     3361 cgttctgtga gctcctatgg aaacatccgt gctgtcgcca cagccaggag cctcaacaaa
     3421 tctttgcaga acctgagttt gacagaggaa tctggtggcg cggtggcgtt ctcccccgga
     3481 aacctcagca ccagcagcag cgccagcagc accctgggca gccccgagaa tgaggagcat
     3541 atcctgtcct tcgagaccat cgacaagatg cgccgcgcca gctcctactc ctccctcaac
     3601 tccctcatcg gagtttcctt taacagtgtt tacactcaga tttggagagt cctgctgcac
     3661 ctggctgctg acccctatcc agaggtctcg gacgtggcca tgaaagtact caacagcatc
     3721 gcctacaagg ccaccgtgaa cgcccggccg cagcgcgtcc tggacacctc ctccctcacg
     3781 cagtcggccc ccgccagccc caccaacaag ggcgtgcaca tccaccaggc ggggggctcc
     3841 cctccggcgt ccagcaccag cagctccagc ctgaccaacg atgtggccaa gcagccggtc
     3901 agccgagact tgccttctgg ccggccgggc accacaggcc ccgctggggc gcagtacacc
     3961 cctcactccc accagttccc ccggacacgg aagatgttcg acaagggccc agagcagact
     4021 gcggacgacg cggacgatgc tgctggacac aaaagtttca tctccgccac ggtgcagacg
     4081 gggttctgcg actggagcgc ccgctatttt gcccagcccg tcatgaagat cccagaagag
     4141 cacgacctgg agagtcagat ccgcaaggag cgggagtggc ggttcctgcg aaacagccgt
     4201 gtcaggaggc aggcccagca agtcattcag aagggcatta cgagattgga cgaccaaata
     4261 tttctgaaca ggaaccccgg cgtcccctct gtggtgaaat tccacccctt cacgccgtgc
     4321 atcgctgtag ccgacaagga cagcatctgc ttttgggact gggagaaagg ggagaagctg
     4381 gattatttcc acaatgggaa ccctcggtac acgagggtca ctgccatgga gtatctgaat
     4441 ggccaggact gctcgcttct gctgacggcc acagacgatg gtgccatcag ggtctggaag
     4501 aattttgctg atttggaaaa gaacccagag atggtgaccg cgtggcaggg gctctcggac
     4561 atgctgccaa cgacgcgagg agctgggatg gtggtggact gggagcagga gaccggcctc
     4621 ctcatgagct caggagacgt gcggatcgtc cggatctggg acacagaccg tgagatgaag
     4681 gtgcaggaca tccctacggg cgcagacagc tgtgtgacga gtctgtcctg tgattcccac
     4741 cgctcactca tcgtggctgg cctcggtgac ggctccatcc gcgtctacga cagaaggatg
     4801 gcactcagcg aatgccgcgt catgacgtac cgggagcaca cagcctgggt ggtgaaggcc
     4861 tccctgcaga agcgtcccga cggccacatc gtgagtgtga gcgtcaatgg agatgtgcgc
     4921 atctttgatc cccggatgcc tgagtcggta aatgtgcttc agatcgtgaa ggggctgacg
     4981 gccctggaca tccaccccca ggcggacctg atcgcatgtg gctccgtcaa tcagttcacc
     5041 gccatctaca acagcagcgg agagctcatc aacaacatca agtactacga cggcttcatg
     5101 ggccagcggg tcggcgccat cagctgcctg gccttccacc cgcactggcc tcacctggcc
     5161 gtgggaagca acgactacta catctccgtg tactcggtgg agaagcgtgt cagatagcgg
     5221 cgtgacccgg gcccaccagg ccacggccgc ctgctgtaca tagtgaagct gtcactcgcc
     5281 ggggcacggg gcgtcggctg ctgcggcccc gcagtgtgaa cgttggctgc tgccttagct
     5341 gctgatgacg gcaggagggc cctgctactc gcttttgtct gtcttcgctg tcgtgtctgg
     5401 aatgtcaggg aaggggaggg ctcgggttga cggtggcttc ccactgagca ccagcatcca
     5461 ggtgcacccc cgcggccacg gcgcctctgt ccctctcctg ttctgtgttt ctctgagacg
     5521 ctgaaagggg aaacacctca ctttatttcc atgtaatcag agcattagct gcagaaaaac
     5581 ccccagacag agccctggcg gagaggcagg cgctggggct cctacgggtc cctggggcag
     5641 ctgtccccat caggccaaga gcgagcgaga ggcgctgccc cagccaggcc caccacctct
     5701 cacagtcagt gcacgcaagc agggacattt cctagccagc tgggggacac tggaaattcg
     5761 ggaaaccaag agagaggaag aaggagacgc ccctccaact ggcgggtgtg aaggaagccg
     5821 cccaggggtc cgggctgtcc ttggccgctg gcagcatcac tgagcaggaa gcgcacagcc
     5881 caccctcccc gcacctccag gtctctggac tccagttttg gcccctctca cacagagctg
     5941 tcagcagggg ccgctgtggc ggtgcacagg ggaggcaggt ccttggcgag gtagcccctg
     6001 ccttaatcca cggggctcct ttccctccga agggctgctc ttccccacag gcgcggggac
     6061 agcagcccga cctgtggtct ccatgcctgt gccctcacac aggtgtagca cacgcatgtg
     6121 cagatggcac cacggccggc acctgggggc acacacatgc aggcggcgtg gtctccctgc
     6181 tctgtcccca cacgttcctc acatacaggc aagaggcact gccgggtccc ggacggctcc
     6241 gggtgacacc agccccgtct ccagccttga gccgcccatg ctgatgcgac ctcggctgac
     6301 agctgggcct gtggtgcaga caggagctgt gtggacagtc ccgcccagga ggggccgcag
     6361 ggcgtgtatg agcagttttg caaacagaac acaaccacaa tgatggtatt ttgaaaagtg
     6421 ttctttccgt gttcgtcggg aatcaggatt attgagaggt gaaggagcca ggtggcttca
     6481 ttctggcggt gagaggccca tgaccacggg agtgagagct ggtgtggcga ggcccggctc
     6541 tcctgcggtg tggctggtgg cctgccgtgg ccaagagcat cttctgggtg gatggaaccc
     6601 tgcctggtca catttggcca gagacacacc tggccctcag ggggctgagc tggagactga
     6661 gctggggctg gccgggacgt gacaaggcag gacagaggcg gcccctccgc tgctcctttt
     6721 tggaatgcga gctcccacca gaagaaggtt ccggcacgaa tcccatcccc acgtctgggc
     6781 cgagaaagca gcccgggtcc ggaaggtgta gagagtcccg gcctcactca gctcacaggg
     6841 cgtgccaggc ggcaacacca gaatcttcca gaagcccagc tccacccgca cacgcagctt
     6901 cccatccagt ccttcaactc aattcttacc caacacgcgt ttccgtttgt tttgagacaa
     6961 aatcaccacc tgtcaaaagg caggtggctc cagaggggtc aagacccccc cccccgcccc
     7021 cgctccaccc tggagcccac ccccatgggc actgcgtgcc gcctgcacgt gggctgtctt
     7081 cacaggtctg atgtgaaaat tcaatcacga cgttaaccgg ctcgagagag cgccggccta
     7141 gaggctcatt atctatttat tttaccaaac gcgaattgag acggactttg acaaaacacg
     7201 aaatggtaat gtgaagctaa gagcagagag tgaccaacag taaacaacac gcgcagactc
     7261 aaaaaaaaaa aaaaaagggc ggccgctaag taagtaagga tccccagctt ggccgccatg
     7321 gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7381 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7441 atcttatcat gtctggatcg ggaattaatt cggcgcagca ccatggcctg aaataacctc
     7501 tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg
     7561 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7621 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7681 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7741 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7801 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7861 ttttggaggc ctaggctttt gcaaaaagct gttaacagct tggcactggc cgtcgtttta
     7921 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     7981 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8041 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     8101 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     8161 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     8221 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     8281 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     8341 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     8401 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     8461 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8521 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     8581 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     8641 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     8701 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     8761 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     8821 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8881 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8941 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9001 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9061 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9121 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9181 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9241 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9301 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9361 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9421 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9481 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9541 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9601 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9661 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9721 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9781 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9841 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9901 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10081 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10141 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10201 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10261 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10321 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10381 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10441 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10501 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10561 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10621 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10681 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10741 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10801 tccccgcgcg ttggccgatt cattaatg
//
