LOCUS       sequence-162822-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Sarbassov DD, Guertin DA, Ali SM, Sabatini DM
  TITLE     Phosphorylation and regulation of Akt/PKB by the rictor-mTOR 
            complex.
  JOURNAL   Science 2005 Feb 18;307(5712):1098-101.
  PUBMED    15718470
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2005-02-18"; volume: "307(5712)"; pages: "1098-101"
COMMENT     Sequence Label: Raptor_2 shRNA
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    135..252
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        301..811
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     730..748
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(833..852)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ggctttatat atcttgtgga aaggacgaaa caccggggct agtctgtttc gaaatttctt
       61 cctgtcaaaa tttcgaaaca gactagcctt tttgaattct cgacctcgag acaaatggca
      121 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      181 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      241 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag
      301 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
      361 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      421 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      481 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      541 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      601 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      661 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      721 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      781 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga
      841 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
      901 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
      961 cgagcgggtc accgagctgc
//
